Phylogenetic analysis of the genera Streptomyces and Kitasatospora based on partial RNA polymerase β-subunit gene (rpoB) sequences
| Autor: | Bum Joon Kim, Chang Yong Cha, Yoon Hoh Kook, Chang-Jin Kim, Sueng Hyun Lee, Young Hwan Koh, Jin Won Hyun, Jongsik Chun |
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| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Genetics biology Phylogenetic tree Streptomycetaceae Molecular Sequence Data DNA-Directed RNA Polymerases General Medicine biology.organism_classification rpoB DNA Ribosomal Microbiology Streptomyces RNA Bacterial Monophyly Sister group Genes Bacterial Phylogenetics RNA Ribosomal 16S Kitasatospora Clade Phylogeny Ecology Evolution Behavior and Systematics DNA Primers |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 54:593-598 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.02941-0 |
| Popis: | The RNA polymerase β-subunit genes (rpoB) of 67 Streptomyces strains, representing 57 species, five Kitasatospora strains and Micromonospora echinospora KCTC 9549 were partially sequenced using a pair of rpoB PCR primers. Among the streptomycetes, 99·7–100 % similarity within the same species and 90·2–99·3 % similarity at the interspecific level were observed by analysis of the determined rpoB sequences. The topology of the phylogenetic tree based on rpoB sequences was similar to that of 16S rDNA. The five Kitasatospora strains formed a stable monophyletic clade and a sister group to the clade comprising all Streptomyces species. Although there were several discrepancies in the details, considerable agreement was found between the results of rpoB analysis and those of numerical phenetic classification. This study demonstrates that analysis of rpoB can be used as an alternative genetic method in parallel to conventional taxonomic methods, including numerical phenetic and 16S rDNA analyses, for the phylogenetic analyses of the genera Streptomyces and Kitasatospora. |
| Databáze: | OpenAIRE |
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