Nutritional impact of low-cost substrates on biphasic fermentation for conidia production of the fungal biopesticide Metarhizium anisopliae
| Autor: | Polyane de Sá Santos, Kauana Abati, Gabriel Moura Mascarin, Italo Delalibera Júnior, Natalie Verónika Rondinel Mendoza |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Environmental Engineering
Bran biology Renewable Energy Sustainability and the Environment Chemistry 020209 energy fungi food and beverages Metarhizium anisopliae Bioengineering 02 engineering and technology 010501 environmental sciences biology.organism_classification 01 natural sciences Spore Conidium 0202 electrical engineering electronic engineering information engineering Blastospore Yeast extract Fermentation Food science Bagasse Waste Management and Disposal RESÍDUOS INDUSTRIAIS 0105 earth and related environmental sciences |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 2589-014X |
| DOI: | 10.1016/j.biteb.2020.100619 |
| Popis: | Agro-industrial wastes (by-products) were used in a biphasic fermentation system for the optimal production of Metarhizium anisopliae conidia. Submerged fermentation carried out with 45 g L−1 glucose and 36 g L−1 yeast extract rendered the highest blastospores yield within four days. Blastospores were used as the inoculum source in solid-state fermentation alternatively to conidia suspension. The solid substrates included rice, bran, and inert supports for growth and sporulation. Pre-cooked rice inoculated with blastospores showed higher yield and faster conidia production compared to inoculation with conidia. The optimal conidia production was obtained after seven days when inoculated with blastospore-based pre-culture. Conidia production after fungal growth on wheat bran (1.2 × 109 conidia g−1) was twice as high as on corn bran. Sugarcane bagasse was the best supporting matrix for growth and sporulation (5.14 × 108 conidia g−1). This study highlights the feasibility of a cost-effective biphasic liquid-solid fermentation of M. anisopliae. |
| Databáze: | OpenAIRE |
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