Shengjiang Powder ameliorates myocardial injury in septic rats by downregulating the phosphorylation of P38-MAPK

Autor: Feng-hua Qian, Yi-ming Qian, Jian Guo, Mengwen Shen, Weiwei Zhang, Chunlei Ding, Lei Zhao
Rok vydání: 2019
Předmět:
Male
0106 biological sciences
Pyridines
p38 mitogen-activated protein kinases
Anti-Inflammatory Agents
Apoptosis
Pharmacology
p38 Mitogen-Activated Protein Kinases
01 natural sciences
General Biochemistry
Genetics and Molecular Biology
Sepsis
Downregulation and upregulation
Proliferating Cell Nuclear Antigen
Animals
Medicine
Myocytes
Cardiac
Phosphorylation
Rats
Wistar
Interleukin 6
biology
Interleukin-6
Tumor Necrosis Factor-alpha
business.industry
Myocardium
Imidazoles
NF-kappa B
General Medicine
medicine.disease
Rats
Proliferating cell nuclear antigen
Disease Models
Animal
Gene Expression Regulation
Heart Injuries
biology.protein
Tumor necrosis factor alpha
Signal transduction
General Agricultural and Biological Sciences
business
Drugs
Chinese Herbal
Signal Transduction
010606 plant biology & botany
Zdroj: Journal of Biosciences. 44
ISSN: 0973-7138
0250-5991
DOI: 10.1007/s12038-019-9857-7
Popis: Sepsis is the systemic inflammatory response caused by infection. Cardiac dysfunction is an acknowledged result of sepsis. Shengjiang Powder, a prescribed traditional Chinese medicine, showed anti-infection and antipyretic functions in our previous study. In this study, we established a septic rat model via cecal ligation puncture (CLP) to evaluate the effects of Shengjiang Powder on sepsis and the involvement of P38 mitogen activated protein kinase (P38-MAPK) signaling. The 10 main ingredients of Shengjiang Powder were identified by LC-MS. The results of this study indicated that Shengjiang Powder at a concentration of 3.0 g/kg with SB203580 (an inhibitor of P38-MAPK) could improve myocardial injury, ameliorate the histopathological abnormalities, decrease apoptosis and upregulate proliferating cell nuclear antigen (PCNA) levels in myocardial tissues. Further, cytokine mRNA expression levels (tumor necrosis factor - alpha, TNF-α and interleukin 6, IL-6) were decreased by Shengjiang Powder and SB203580 in the myocardial tissues. Furthermore, the p-P38 protein level in myocardial tissues was upregulated in septic rats but decreased upon treatment with Shengjiang Powder and SB203580; however, the relative protein level of P38 showed no significant changes. Collectively, Shengjiang Powder showed a myocardial protective effect on rats with CLP-induced sepsis.
Databáze: OpenAIRE
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