Microbial engineering strategies to improve cell viability for biochemical production
| Autor: | Tat-Ming Lo, Matthew Wook Chang, Hua Ling, Wei Suong Teo, Binbin Chen, Aram Kang |
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| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Cell Survival Cell Membrane Cell Bioengineering Biology Applied Microbiology and Biotechnology Cell membrane Metabolic engineering Synthetic biology Enzyme medicine.anatomical_structure Metabolic Engineering chemistry Biochemistry Biofuels Escherichia coli medicine Humans Viability assay Resource consumption Intracellular Biotechnology |
| Zdroj: | Biotechnology Advances. 31:903-914 |
| ISSN: | 0734-9750 |
| DOI: | 10.1016/j.biotechadv.2013.02.001 |
| Popis: | Efficient production of biochemicals using engineered microbes as whole-cell biocatalysts requires robust cell viability. Robust viability leads to high productivity and improved bioprocesses by allowing repeated cell recycling. However, cell viability is negatively affected by a plethora of stresses, namely chemical toxicity and metabolic imbalances, primarily resulting from bio-synthesis pathways. Chemical toxicity is caused by substrates, intermediates, products, and/or by-products, and these compounds often interfere with important metabolic processes and damage cellular infrastructures such as cell membrane, leading to poor cell viability. Further, stresses on engineered cells are accentuated by metabolic imbalances, which are generated by heavy metabolic resource consumption due to enzyme overexpression, redistribution of metabolic fluxes, and impaired intracellular redox state by co-factor imbalance. To address these challenges, herein, we discuss a range of key microbial engineering strategies, substantiated by recent advances, to improve cell viability for commercially sustainable production of biochemicals from renewable resources. |
| Databáze: | OpenAIRE |
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