Combined effects of insulin-like growth factor-1 and transforming growth factor-β1 on periosteal mesenchymal cells during chondrogenesis in vitro
| Autor: | J. W. Sperling, Shawn W. O'Driscoll, T. Fukumoto, Cheryl A. Conover, James S. Fitzsimmons, A. Sanyal, G. G. Reinholz |
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| Rok vydání: | 2003 |
| Předmět: |
Cartilage
Articular IGF-1 TGF-β1 Periosteum Proliferation Chondrogenesis medicine.medical_specialty medicine.medical_treatment Biomedical Engineering Type II collagen In situ hybridization In Vitro Techniques Organ culture Transforming Growth Factor beta1 Insulin-like growth factor Rheumatology Transforming Growth Factor beta Periosteum Internal medicine medicine Animals Orthopedics and Sports Medicine Insulin-Like Growth Factor I Collagen Type II Chemistry Cartilage Mesenchymal stem cell Chondrogenesis Hindlimb Cell biology medicine.anatomical_structure Endocrinology Rabbits Transforming growth factor |
| Zdroj: | Osteoarthritis and Cartilage. 11:55-64 |
| ISSN: | 1063-4584 |
| DOI: | 10.1053/joca.2002.0869 |
| Popis: | Objective Periosteum contains undifferentiated mesenchymal stem cells that have both chondrogenic and osteogenic potential, and has been used to repair articular cartilage defects. During this process, the role of growth factors that stimulate the periosteal mesenchymal cells toward chondrogenesis to regenerate articular cartilage and maintain its phenotype is not yet fully understood. In this study, we examined the effects of insulin-like growth factor-1 (IGF-1) and transforming growth factor-β1 (TGF-β1), alone and in combination, on periosteal chondrogenesis using an in vitro organ culture model. Methods Periosteal explants from the medial proximal tibia of 2-month-old rabbits were cultured in agarose under serum free conditions for up to 6 weeks. After culture the explants were weighed, assayed for cartilage production via Safranin O staining and histomorphometry, assessed for proliferation via proliferative cell nuclear antigen (PCNA) immunostaining, and assessed for type II collagen mRNA expression via in situ hybridization. Results IGF-1 significantly increased chondrogenesis in a dose-dependent manner when administered continuously throughout the culture period. Continuous IGF-1, in combination with TGF-β1 for the first 2 days, further enhanced overall total cartilage growth. Immunohistochemistry for PCNA revealed that combining IGF-1 with TGF-β1 gave the strongest proliferative stimulus early during chondrogenesis. In situ hybridization for type II collagen showed that continuous IGF-1 maintained type II collagen mRNA expression throughout the cambium layer from 2 to 6 weeks. Conclusion The results of this study demonstrate that IGF-1 and TGF-β1 can act in combination to regulate proliferation and differentiation of periosteal mesenchymal cells during chondrogenesis. Copyright 2003 OsteoArthritis Research Society International. Published by Elsevier Science Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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