Purification and Cloning of Aggrecanase-1: A Member of the ADAMTS Family of Proteins

Autor:	Rui-Qin Liu, Timothy Burn, Kathleen Murphy, Gregory Hollis, Stuart A. Rosenfeld, Barbara H. Wiswall, Robert A. Copeland, Yoshifumi Itoh, Carl P. Decicco, Hideaki Nagase, F. Yang, Robert C. Bruckner, Robert C. Newton, James M. Trzaskos, Henry J. George, Richard Wynn, J. L. Duke, Ronald L. Magolda, Dawn Ellis, Michael A. Pratta, A. Rockwell, Ilgar Abbaszade, E. C. Arner, Harold D. Ross, M.C. Hillman, Micky D. Tortorella, Kimberly A. Solomon, Jeannine M. Hollis
Rok vydání:	1999
Předmět:	Disintegrins Molecular Sequence Data Protein Sorting Signals Biology Hydroxamic Acids ADAMTS1 Protein Catalytic Domain Humans Lectins C-Type Protease Inhibitors Aggrecans Amino Acid Sequence Cloning Molecular Aggrecan Aggrecanase Extracellular Matrix Proteins Metalloproteinase Multidisciplinary Arthritis ADAMTS Metalloendopeptidases ADAMTS4 Protein ADAM Proteins Recombinant Proteins Cartilage ADAMTS4 Biochemistry Proteoglycans Procollagen N-Endopeptidase Sequence Analysis ADAMTS Proteins Interleukin-1
Zdroj:	Science. 284:1664-1666
ISSN:	1095-9203 0036-8075
Popis:	We purified, cloned, and expressed aggrecanase, a protease that is thought to be responsible for the degradation of cartilage aggrecan in arthritic diseases. Aggrecanase-1 [a disintegrin and metalloproteinase with thrombospondin motifs–4 (ADAMTS-4)] is a member of the ADAMTS protein family that cleaves aggrecan at the glutamic acid-373–alanine-374 bond. The identification of this protease provides a specific target for the development of therapeutics to prevent cartilage degradation in arthritis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fc2a5018fca3fd44f5c1740c2430b66 https://doi.org/10.1126/science.284.5420.1664 Zobrazit plný text záznamu Plný text ve formátu PDF