Molecular xenomonitoring (MX) and transmission assessment survey (TAS) of lymphatic filariasis elimination in two villages, Menoufyia Governorate, Egypt
| Autor: | M. A. Moustafa, H. S. Thabet, D. M. Hamdy, R. A. Tawfik, M. M. I. Salamah, M. M. Mehrez |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) Veterinary medicine 030106 microbiology 030231 tropical medicine Mosquito Vectors Elephantiasis Real-Time Polymerase Chain Reaction Chromatography Affinity law.invention 03 medical and health sciences Elephantiasis Filarial 0302 clinical medicine law Surveys and Questionnaires parasitic diseases Culex pipiens Disease Transmission Infectious Animals Humans Medicine Wuchereria bancrofti Child Mass drug administration Lymphatic filariasis biology business.industry General Medicine medicine.disease biology.organism_classification Cross-Sectional Studies Filaricides Infectious Diseases Transmission (mechanics) Mass Drug Administration Egypt Female business Disease transmission Filarial antigen |
| Zdroj: | European Journal of Clinical Microbiology & Infectious Diseases. 36:1143-1150 |
| ISSN: | 1435-4373 0934-9723 |
| DOI: | 10.1007/s10096-017-2901-3 |
| Popis: | Lymphatic filariasis (LF) is focally endemic in Egypt where the female mosquito, Culex pipiens, is responsible for its transmission. The aim of the study was to investigate the impact of implementation of the 13th round of MDA in two Egyptian villages in the Menoufyia Governorate area after failing the transmission assessment survey (TAS) in 2005 using two methods, and to decide whether it is safe to stop MDA in these, as well as in similar implementation units (IUs). To achieve this aim, both the immunochromatographic card test (ICT) and molecular xenomonitoring (MX) techniques were employed. A cross-sectional study was carried out in the villages in 2014 with two sections: Section (1): a school-based survey where all the primary school entrants (6-7) years of age were tested by ICT. Section (2): a mosquito-based survey where a total of 152 mosquito pools collected from Samalay and 167 from Kafr El-Tarainah were tested for the presence of the gDNA of Wuchereria bancrofti microfilaria by real-time PCR assays. The results revealed that all primary school children in both villages were 100% negative for antigenemia. Also, all mosquito pools were 100% negative for the microfilarial gDNA. |
| Databáze: | OpenAIRE |
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