Attenuation artifacts in light sheet fluorescence microscopy corrected by OPTiSPIM
| Autor: | Jürgen Mayer, Alexandre Robert-Moreno, Jim Swoger, James Sharpe |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
lcsh:Applied optics. Photonics
0301 basic medicine Mesoscopic physics Materials science genetic structures business.industry Attenuation lcsh:TA1501-1820 computer.software_genre Sample (graphics) Optical projection tomography Article Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials 03 medical and health sciences 030104 developmental biology Optics Transmission (telecommunications) Voxel Light sheet fluorescence microscopy Shadow lcsh:QC350-467 business computer lcsh:Optics. Light |
| Zdroj: | Light, Science & Applications Recercat. Dipósit de la Recerca de Catalunya instname Light: Science & Applications, Vol 7, Iss 1, Pp 1-13 (2018) |
| Popis: | Light sheet fluorescence microscopy (LSFM) is rapidly becoming an essential technology for mesoscopic imaging of samples such as embryos and adult mouse organs. However, LSFM can suffer from optical artifacts for which there is no intrinsic solution. The attenuation of light due to absorbing material causes “shadow” artifacts along both the illumination and detection paths. Several approaches have been introduced to reduce this problem, including scanning illumination and multi-view imaging. However, neither of these approaches completely eliminates the problem. If the distribution of the absorbing material is complex, shadows cannot be avoided. We introduce a new approach that relies on multi-modal integration of two very different mesoscopic techniques. Unlike LSFM, optical projection tomography (OPT) can operate in transmission mode to create a voxel map of the 3D distribution of the sample’s optical attenuation. Here, we demonstrate a hybrid instrument (OPTiSPIM) that can quantify this attenuation and use the information to correct the shadow artifacts of LSFM. Imaging: Biological imaging technique comes out from the shadows Scientists in Spain have developed a new method that combines two different techniques for improving the quality of images produced by light sheet fluorescence microscopy (LSFM), a tool that has revolutionized the imaging of biological samples. Although LSFM is a becoming an increasingly essential tool in biological imaging, it suffers from ‘shadows’ or ‘stripe artifacts’ when samples contain regions that significantly attenuate light, such as eye pigmentation. Now, Jim Swoger and colleagues from the Center for Genomic Regulation in Barcelona have developed a technique that measures the attenuation and uses three-dimensional mapping to correct for artifacts. By combining Optical Projection Tomography and multi-view LSFM imaging modalities into a single hybrid system, called OPTiSPIM, the researchers have developed a method that produces more accurate images of biological materials, such as mice and rodent embryos and organs. |
| Databáze: | OpenAIRE |
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