Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients
| Autor: | Srdjan Stojanović, Zorica Stevic, David R. Jones, Slobodan Apostolski, Sanja Pavlovic, Duško Blagojević, Mihajlo B. Spasić, Vesna Niketić, Aleksandra Nikolić Kokić |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male medicine.medical_specialty Physiology Iron Clinical Biochemistry Hydroxylamine Nitric Oxide Biochemistry cerebrospinal fluid Nitric oxide Pathogenesis Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cerebrospinal fluid uric acid nitric oxide Internal medicine parasitic diseases medicine Humans Amyotrophic lateral sclerosis Dinitrosyl iron complex Nitrites Aged 030304 developmental biology 0303 health sciences biology Superoxide Dismutase Amyotrophic Lateral Sclerosis amyotrophic lateralsclerosis Biochemistry (medical) Cell Biology Middle Aged medicine.disease 3. Good health Endocrinology chemistry biology.protein Uric acid Female Nitrogen Oxides Oxidation-Reduction 030217 neurology & neurosurgery Ex vivo |
| Zdroj: | Redox Report |
| ISSN: | 1743-2928 1351-0002 |
| DOI: | 10.1179/135100005x70242 |
| Popis: | Recent findings indicate that nitric oxide (NOcenter dot) over-production might be an important factor in the pathogenesis of sporadic amyotrophic lateral sclerosis (SALS). We measured significantly higher concentrations of uric acid and thiol group-containing molecules (R-SH groups) in the cerebrospinal fluid (CSF) from SALS patients compared to controls. The above factors, together with a slightly increased free iron concentration found in the CSF, favour conditions necessary for the formation of the dinitrosyl iron complex, capable of NOcenter dot bio-transformation. Thus, we performed ex vivo saturation of CSF ( from both SALS patients and controls) with NOcenter dot. A decrease in the level of R - SH was found. This was more pronounced in the CSF from SALS patients. In the CSF from SALS patients the production of nitrite and hydroxylamine was greater than that observed in the CSF from controls. Moreover, we also found increased Cu, Zn-SOD activity in the CSF from SALS patients ( when compared to control subjects) but no activity corresponding to Mn-SOD in any CSF samples. As Cu, Zn-SOD can react with nitroxyl forming NOcenter dot, the conditions for a closed, but continuous, loop of NOcenter dot biotransformation are present in the CSF of ALS patients. null |
| Databáze: | OpenAIRE |
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