Sex‐specific effects of leptin administration to pregnant mice on the placentae and the metabolic phenotypes of offspring
| Autor: | N. M. Bazhan, Elena I. Denisova, Elena N. Makarova, V. V. Kozhevnikova |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty mice placenta Offspring Biology leptin General Biochemistry Genetics and Molecular Biology Energy homeostasis 03 medical and health sciences chemistry.chemical_compound Fetus 0302 clinical medicine developmental programming Placenta Adipocyte Internal medicine medicine Animals Obesity lcsh:QH301-705.5 Research Articles Sex Characteristics Pregnancy Leptin medicine.disease Recombinant Proteins Mice Inbred C57BL Phenotype 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry lcsh:Biology (General) Hyperglycemia 030220 oncology & carcinogenesis Gestation Female pregnancy Research Article |
| Zdroj: | FEBS Open Bio, Vol 10, Iss 1, Pp 96-106 (2020) FEBS Open Bio |
| ISSN: | 2211-5463 |
| Popis: | Obesity during pregnancy has been shown to increase the risk of metabolic diseases in the offspring. However, the factors within the maternal milieu which affect offspring phenotypes and the underlying mechanisms remain unknown. The adipocyte hormone leptin plays a key role in regulating energy homeostasis and is known to participate in sex‐specific developmental programming. To examine the action of leptin on fetal growth, placental gene expression and postnatal offspring metabolism, we injected C57BL mice with leptin or saline on gestational day 12 and then measured body weights (BWs) of offspring fed on a standard or obesogenic diet, as well as mRNA expression levels of insulin‐like growth factors and glucose and amino acid transporters. Male and female offspring born to leptin‐treated mothers exhibited growth retardation before and a growth surge after weaning. Mature male offspring, but not female offspring, exhibited increased BWs on a standard diet. Leptin administration prevented the development of hyperglycaemia in the obese offspring of both sexes. The placentas of the male and female foetuses differed in size and gene expression, and leptin injection decreased the fetal weights of both sexes, the placental weights of the male foetuses and placental gene expression of the GLUT1 glucose transporter in female foetuses. The data suggest that mid‐pregnancy is an ontogenetic window for the sex‐specific programming effects of leptin, and these effects may be exerted via fetal sex‐specific placental responses to leptin administration. Leptin administration to pregnant mice transiently inhibited growth rate in the foetuses of both sexes, decreased placental GLUT1 mRNA level only in female foetuses, and decreased placental weight and increased growth rate after weaning only in male offspring. Sex‐specific programming effect of leptin may be related to its different action on the placentas of male and female foetuses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text