Assessment of TCF7L2 expression after bariatric surgery
| Autor: | Carlos Eduardo S. Macedo, Guilherme da Conti, Álvaro Antônio Bandeira Ferraz, Andriu dos Santos Catena, Fernando Santa Cruz, Clarissa Guedes Noronha, Danyelly Bruneska, Malu Rosa |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Physiology Gene Expression Bariatric Surgery 030105 genetics & heredity Body Mass Index Endocrinology Mathematical and Statistical Techniques 0302 clinical medicine Medicine and Health Sciences Postoperative Period Prospective Studies Prospective cohort study Multidisciplinary Statistics Regression analysis Middle Aged Treatment Outcome Physiological Parameters Physical Sciences Regression Analysis Medicine Female Transcription Factor 7-Like 2 Protein Research Article Adult medicine.medical_specialty Adolescent Endocrine Disorders Science Surgical and Invasive Medical Procedures 030209 endocrinology & metabolism Linear Regression Analysis Research and Analysis Methods Positive correlation Digestive System Procedures Young Adult 03 medical and health sciences Extraction techniques Weight Loss Linear regression Diabetes Mellitus Genetics medicine Humans Obesity Statistical Methods Aged business.industry Body Weight Biology and Life Sciences nutritional and metabolic diseases Type 2 Diabetes Mellitus RNA extraction Peripheral blood Surgery Diabetes Mellitus Type 2 Metabolic Disorders business TCF7L2 Body mass index Mathematics |
| Zdroj: | PLoS ONE, Vol 14, Iss 5, p e0216627 (2019) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0216627 |
| Popis: | ObjectiveTo assess the influence of bariatric surgery on transcription factor 7-like 2 (TCF7L2) expression and its association with body mass index (BMI) and Type 2 diabetes mellitus (T2DM).MethodsProspective study performed between 2016 and 2018, where 26 obese patients undergoing bariatric surgery were divided into two subgroups: diabetics and non-diabetics. The RNAs were extracted from peripheral blood samples that were obtained from each patient in two different moments: before surgery and after 12 months of follow-up. The relative expression of TCF7L2 was determined according to the delta-Ct method.ResultsThe linear regression model of BMI x delta-Ct showed a positive correlation (p = 0.037). In the subgroups, an inversely proportional relationship was found between delta-Ct and BMI in the diabetic group and a directly proportional relationship in the non-diabetic group (p>0.05 in both). In the postoperative period, the regression model was similar to the preoperative, except when analyzing the subgroups, where diabetic patients showed a directly proportional relationship (p>0.05). The relative expression of TCF7L2 showed an average of 1.16 ± 0.91, CI-95% 0.79-1.53. There was an increase in relative expression of 48% in the non-diabetic group (p = 0.021), and a decrease of 27% in the T2DM group (p>0.05) in the postoperative. There was a positive correlation between a greater decrease in BMI and increased relative expression (p = 0.027).ConclusionOur results showed that generally, the TCF7L2 expression increase with a decrease in BMI, however, for patients with T2DM, it exhibits an inverse pattern, which is normalized one year after bariatric surgery. |
| Databáze: | OpenAIRE |
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