Molecular cloning, characterization and expression profiling of galectin-9 gene from Labeo rohita (Hamilton, 1822)
| Autor: | Kurcheti Pani Prasad, Megha Kadam Bedekar, Annam Pavan Kumar, Rahul Krishnan, Zahoor Mushtaq |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Fish Proteins
0301 basic medicine animal structures Galectins Cyprinidae Danio Aquatic Science Fish Diseases 03 medical and health sciences Immune system Gene expression Animals Environmental Chemistry Amino Acid Sequence Phylogeny Galectin Innate immune system Base Sequence biology Gene Expression Profiling 04 agricultural and veterinary sciences General Medicine biology.organism_classification Molecular biology Immunity Innate Aeromonas hydrophila Labeo Gene expression profiling 030104 developmental biology Gene Expression Regulation 040102 fisheries 0401 agriculture forestry and fisheries Gram-Negative Bacterial Infections Sequence Alignment |
| Zdroj: | Fish & Shellfish Immunology. 76:287-292 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2018.02.037 |
| Popis: | Galectin-9 is a b-galactoside-binding tandem repeat galectin that regulates many cellular functions, ranging from cell adhesion to pathogen recognition. In spite of extensive study of mammalian galectin importance in immune system, little is known about that of fish. To study the normal expression and immune response of Labeo rohita to pathogens, a tandem-repeat galectin-9 from Labeo rohita was identified and named LrGal-9. Its full-length cDNA was 1534 bp encoded 291 amino acids (35.12 KDa), shared the highest 81% identity with the galectin-9 of Danio rerio. LrGal-9 identified in this study lacked signal peptide and a transmembrane domain like galectin-9 members reported in other fishes. Quantitative PCR showed that LrGal-9 was lowly expressed in gill, muscle, heart, highly expressed in tested immune tissues (intestine, kidney, liver, spleen) in normal body. After Aeromonas hydrophila challenge, LrGal-9 was remarkably increased in all tested immune tissues in a time-dependent manner. These results suggest that LrGal-9 plays a role in innate immunity in Labeo rohita. |
| Databáze: | OpenAIRE |
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