A modular microfluidic system based on a multilayered configuration to generate large-scale perfusable microvascular networks
| Autor: | Tao Yue, Da Zhao, Abraham P. Lee, Zayn Biviji, Christopher C.W. Hughes, Xiaolin Wang, Joshua Jonghyun Park, Duc T. T. Phan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Flexibility (anatomy)
Materials science Capillary action Materials Science (miscellaneous) 1.1 Normal biological development and functioning Microfluidics Bioengineering 02 engineering and technology lcsh:Technology Industrial and Manufacturing Engineering Article 03 medical and health sciences chemistry.chemical_compound Engineering Underpinning research Vertical direction medicine Electrical and Electronic Engineering 030304 developmental biology 0303 health sciences Polydimethylsiloxane business.industry lcsh:T Modular design 021001 nanoscience & nanotechnology Condensed Matter Physics Atomic and Molecular Physics and Optics medicine.anatomical_structure chemistry lcsh:TA1-2040 Scalability 0210 nano-technology business lcsh:Engineering (General). Civil engineering (General) Communication channel Biomedical engineering Biotechnology |
| Zdroj: | Microsystems & Nanoengineering, Vol 7, Iss 1, Pp 1-13 (2021) Microsystems & nanoengineering, vol 7, iss 1 Microsystems & Nanoengineering |
| ISSN: | 2055-7434 |
| Popis: | The vascular network of the circulatory system plays a vital role in maintaining homeostasis in the human body. In this paper, a novel modular microfluidic system with a vertical two-layered configuration is developed to generate large-scale perfused microvascular networks in vitro. The two-layer polydimethylsiloxane (PDMS) configuration allows the tissue chambers and medium channels not only to be designed and fabricated independently but also to be aligned and bonded accordingly. This method can produce a modular microfluidic system that has high flexibility and scalability to design an integrated platform with multiple perfused vascularized tissues with high densities. The medium channel was designed with a rhombic shape and fabricated to be semiclosed to form a capillary burst valve in the vertical direction, serving as the interface between the medium channels and tissue chambers. Angiogenesis and anastomosis at the vertical interface were successfully achieved by using different combinations of tissue chambers and medium channels. Various large-scale microvascular networks were generated and quantified in terms of vessel length and density. Minimal leakage of the perfused 70-kDa FITC-dextran confirmed the lumenization of the microvascular networks and the formation of tight vertical interconnections between the microvascular networks and medium channels in different structural layers. This platform enables the culturing of interconnected, large-scale perfused vascularized tissue networks with high density and scalability for a wide range of multiorgan-on-a-chip applications, including basic biological studies and drug screening. |
| Databáze: | OpenAIRE |
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