DNA damaging properties of single walled carbon nanotubes in human colon carcinoma cells
| Autor: | Doris Marko, Anja Rechel, Joanna Pelka, Manfred M. Kappes, Christian G. Hartinger, Frank Hennrich, Helge Gehrke |
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| Rok vydání: | 2011 |
| Předmět: |
DNA damage
Blotting Western Biomedical Engineering Biology Toxicology medicine.disease_cause Microscopy Atomic Force Cell Line chemistry.chemical_compound Mice Lactate dehydrogenase medicine Animals Humans Phosphorylation Cell Proliferation chemistry.chemical_classification Reactive oxygen species Micronucleus Tests L-Lactate Dehydrogenase Cell growth Nanotubes Carbon Glutathione Molecular biology Comet assay Biochemistry chemistry Micronucleus test Colonic Neoplasms Comet Assay Reactive Oxygen Species HT29 Cells Oxidative stress DNA Damage |
| Zdroj: | Nanotoxicology. 7(1) |
| ISSN: | 1743-5404 |
| Popis: | Single walled carbon nanotubes were studied with respect to cytotoxic and genotoxic properties in cells of the gastrointestinal tract as exemplified for the human colon carcinoma cell line HT29. No effect on cell growth in the sulphorhodamine B assay was observed after 24 h of incubation, whereas growth inhibitory properties were found after 48 and 72 h. After 24 h incubation a decrease of mitochondrial activity (WST-1) was measured (≥0.1 μg/ml), whereas membrane integrity (lactate dehydrogenase) was not affected. In cytotoxic concentrations, the formation of reactive oxygen species and a slight increase of total glutathione and nuclear Nrf2 were observed. However, already in subcytotoxic concentrations substantial DNA damaging effects were found in the alkaline comet assay, which were not associated with enhanced formation of formamidopyrimidine-DNA-glycosylase-sensitive sites. In addition, an increase of kinetochore-negative micronuclei (V79) and phosphorylation of the tumour suppressor protein p53 (HT29) underlined the genotoxic potential of these nanostructures. |
| Databáze: | OpenAIRE |
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