In [i]Entamoeba histolytica[/i], a BspA family protein is required for chemotaxis toward tumour necrosis factor
| Autor: | Roman Thibeaux, Patricia Berthon, Nancy Guillén, Aurélie Plaze, Elisabeth Labruyère, Anne Silvestre |
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| Přispěvatelé: | UR Infectiologie animale et Santé publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Unité Biologie Cellulaire du Parasitisme, U786, Institut National de la Santé et de la Recherche Médicale (INSERM), National French Research Agency (ANR, grant MIE-08, Intestinalamibe) - French Parasitology consortium Labex ParaFrap (grant ANR-11-LABX0024) - Institut Pasteur (grant PTR 391) - INRA Infectiologie et Santé Publique ISP Cluster (grant 32000266), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Biologie Cellulaire du Parasitisme, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), ANR-11-LABX-0024,ParaFrap,Alliance française contre les maladies parasitaires(2011), ANR-08-MIEN-0017,INTESTINALAMIBE,Analyse moléculaire et cellulaire de la mobilité et du processus infectieux de Entamoeba histolytica pendant l'amibiase(2008), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Guillén, Nancy |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
microscopie confocale
Cell entamoeba histolytica tumour necrosis factor chemotaxi BspA protein Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Microbiology Applied Microbiology and Biotechnology Entamoeba histolytica intestin Virology séquençage parasitic diseases Genetics medicine chimiotaxie Secretion chemotaxis Receptor bioinformatique Molecular Biology lcsh:QH301-705.5 analyse histologique facteur de nécrose tumorale Microbiology and Parasitology RNA Cell migration Chemotaxis Cell Biology biology.organism_classification Molecular biology Microbiologie et Parasitologie 3. Good health Cell biology medicine.anatomical_structure [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology lcsh:Biology (General) protéine amibiase Parasitology Tumor necrosis factor alpha humain |
| Zdroj: | Microbial Cell Microbial Cell, Shared Science Publishers OG, 2015, 2 (7), pp.219-230. ⟨10.15698/mic2015.07.214⟩ Microbial Cell, 2015, 2 (7), pp.219-230. ⟨10.15698/mic2015.07.214⟩ Microbial Cell, Vol 2, Iss 7, Pp 235-246 (2015) Microbial Cell 7 (2), 219-230. (2015) |
| ISSN: | 2311-2638 |
| Popis: | Background: Entamoeba histolytica cell migration is essential for the development of human amoebiasis (an infectious disease characterized by tissue invasion and destruction). The tissue inflammation associated with tumour necrosis factor (TNF) secretion by host cells is a well-documented feature of amoebiasis. Tumour necrosis factor is a chemoattractant for E. histolytica, and the parasite may have a TNF receptor at its cell surface. Methods: confocal microscopy, RNA Sequencing, bioinformatics, RNA antisense techniques and histological analysis of human colon explants were used to characterize the interplay between TNF and E. histolytica. Results: an antibody against human TNF receptor 1 (TNFR1) stained the E. histolytica trophozoite surface and (on immunoblots) binds to a 150-kDa protein. Proteome screening with the TNFR1 sequence revealed a BspA family protein in E. histolytica that carries a TNFR signature domain and six leucine-rich repeats (named here as "cell surface protein", CSP, in view of its cellular location). Cell surface protein shares structural homologies with Toll-Like receptors, colocalizes with TNF and is internalized in TNF-containing vesicles. Reduction of cellular CSP levels abolished chemotaxis toward TNF and blocked parasite invasion of human colon. Conclusions: there is a clear link between TNF chemotaxis, CSP and pathogenesis. |
| Databáze: | OpenAIRE |
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