Structural characterization of four different naturally occurring porcine collagen membranes suitable for medical applications

Autor: Helga Maria Mogel, Michael Hubert Stoffel, Susanne Küker, Niklaus G Stiefel, Thimo Maurer, Jasmin Balmer, Yury Belyaev, Beatriz Vidondo, Birgit Schäfer
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Swine
Physiology
lcsh:Medicine
Biocompatible Materials
02 engineering and technology
Biochemistry
Fluorescence Microscopy
Medicine and Health Sciences
Electron Microscopy
610 Medicine & health
lcsh:Science
Mammals
Microscopy
Multidisciplinary
Decellularization
biology
Optical Imaging
Eukaryota
Light Microscopy
Biomaterial
Heart
021001 nanoscience & nanotechnology
Electrophysiology
Membrane
medicine.anatomical_structure
Vertebrates
Collagen
Scanning Electron Microscopy
Anatomy
0210 nano-technology
Pericardium
Elastic fiber
Research Article
Morphology (linguistics)
Research and Analysis Methods
Fibril
Membrane Potential
03 medical and health sciences
medicine
Animals
Bone regeneration
lcsh:R
Organisms
Biology and Life Sciences
Proteins
Membranes
Artificial
Elastin
Gastrointestinal Tract
030104 developmental biology
Amniotes
Cardiovascular Anatomy
biology.protein
Biophysics
570 Life sciences
lcsh:Q
Collagens
Digestive System
Zdroj: PLoS ONE, Vol 13, Iss 10, p e0205027 (2018)
Maurer, Thimo; Stoffel, Michael Hubert; Belyaev, Yury; Stiefel, Niklaus G; Vidondo, Beatriz; Küker, Susanne; Mogel, Helga Maria; Schäfer, Birgit; Balmer, Jasmin (2018). Structural characterization of four different naturally occurring porcine collagen membranes suitable for medical applications. PLoS ONE, 13(10), e0205027. Public Library of Science 10.1371/journal.pone.0205027
PLoS ONE
ISSN: 1932-6203
Popis: Collagen is the main structural element of connective tissues, and its favorable properties make it an ideal biomaterial for regenerative medicine. In dental medicine, collagen barrier membranes fabricated from naturally occurring tissues are used for guided bone regeneration. Since the morphological characteristics of collagen membranes play a crucial role in their mechanical properties and affect the cellular behavior at the defect site, in-depth knowledge of the structure is key. As a base for the development of novel collagen membranes, an extensive morphological analysis of four porcine membranes, including centrum tendineum, pericardium, plica venae cavae and small intestinal submucosa, was performed. Native membranes were analyzed in terms of their thickness. Second harmonic generation and two-photon excitation microscopy of the native membranes showed the 3D architecture of the collagen and elastic fibers, as well as a volumetric index of these two membrane components. The surface morphology, fiber arrangement, collagen fibril diameter and D-periodicity of decellularized membranes were investigated by scanning electron microscopy. All the membrane types showed significant differences in thickness. In general, undulating collagen fibers were arranged in stacked layers, which were parallel to the membrane surface. Multiphoton microscopy revealed a conspicuous superficial elastic fiber network, while the elastin content in deeper layers varied. The elastin/collagen volumetric index was very similar in the investigated membranes and indicated that the collagen content was clearly higher than the elastin content. The surface of both the pericardium and plica venae cavae and the cranial surface of the centrum tendineum revealed a smooth, tightly arranged and crumpled morphology. On the caudal face of the centrum tendineum, a compact collagen arrangement was interrupted by clusters of circular discontinuities. In contrast, both surfaces of the small intestinal submucosa were fibrous, fuzzy and irregular. All the membranes consisted of largely uniform fibrils displaying the characteristic D-banding. This study reveals similarities and relevant differences among the investigated porcine membranes, suggesting that each membrane represents a unique biomaterial suitable for specific applications.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje