Simple Sequence Repeat (SSR) Markers Linked to the Ligon Lintless (Li1) Mutant in Cotton
Autor: | Sukumar Saha, Allan Zipf, J. N. Jenkins, Russell J. Kohel, Mehmet Karaca, David M. Stelly |
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Rok vydání: | 2002 |
Předmět: |
Genetic Markers
Genetics Gossypium education.field_of_study Genetic Linkage Population Mutant Chromosome Mapping food and beverages Locus (genetics) Biology Chromosomes Plant Plant Leaves Genetic distance Genetic marker Genetic linkage Seeds Genotype education Molecular Biology Chromosome 22 Genetics (clinical) Repetitive Sequences Nucleic Acid Biotechnology |
Zdroj: | Journal of Heredity. 93:221-224 |
DOI: | 10.1093/jhered/93.3.221 |
Popis: | Ligon lintless (Li(1)) is a monogenic, dominant mutant in cotton, whose expression results in extreme reductions in fiber length on mature seed. The objectives of this research were to compare fiber initiation between the Li(1) mutant and TM-1 to reveal the fiber initiation differences between normal and mutant phenotypes, to develop a linkage map of simple sequence repeat (SSR) markers with the Li(1) locus, and to identify the chromosomal location of the Li(1) locus. Comparative scanning electron microscopy studies of fiber development in a normal TM-1 genotype and the near-isogenic Li(1) mutant at 1 and 3 days postanthesis revealed little differences between the two during early stages of development, suggesting that Li(1) gene expression occurs later, probably during the elongation phase. Thirty-eight SSR loci were found to be polymorphic between TM-1 and Li(1) and were used for mapping in an F(2) population. Twenty-two SSR loci, along with Li(1), were located on eight linkage groups, covering a total genetic distance of 218.3 cM. Analysis of individual monosomic and monotelodisomic plants indicated that two SSR loci (MP4030 and MP673) from the Li(1) linkage group were located on chromosome 22. |
Databáze: | OpenAIRE |
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