Responses to K deficiency and waterlogging interact via respiratory and nitrogen metabolism
| Autor: | Cyril Abadie, Adam J. Carroll, Jing Cui, Emmanuelle Lamade, Guillaume Tcherkez |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology F62 - Physiologie végétale - Croissance et développement Plant Science Nitrate 01 natural sciences chemistry.chemical_compound Photosynthesis 2. Zero hunger food and beverages Helianthus annuus Carence du sol Horticulture Metabolome Helianthus Waterlogging (agriculture) P33 - Chimie et physique du sol Nitrogen F60 - Physiologie et biochimie végétale Azote Cell Respiration Métabolite Engorgement du sol 03 medical and health sciences Stress Physiological Respiration Nitrogen cycle Catabolism Water Plant Leaves Citric acid cycle 030104 developmental biology chemistry 13. Climate action Potassium Carence minérale Flux (metabolism) 010606 plant biology & botany |
| Zdroj: | Plant, Cell and Environment |
| ISSN: | 1365-3040 0140-7791 |
| DOI: | 10.1111/pce.13450 |
| Popis: | K deficiency and waterlogging are common stresses that can occur simultaneously and impact on crop development and yield. They are both known to affect catabolism, with rather opposite effects: inhibition of glycolysis and higher glycolytic fermentative flux, respectively. But surprisingly, the effect of their combination on plant metabolism has never been examined precisely. Here, we applied a combined treatment (K availability and waterlogging) to sunflower (Helianthus annuus L.) plants under controlled greenhouse conditions and performed elemental quantitation, metabolomics, and isotope analyses at different sampling times. Whereas separate K deficiency and waterlogging caused well-known effects such as polyamines production and sugar accumulation, respectively, waterlogging altered K-induced respiration enhancement (via the C5 -branched acid pathway) and polyamine production, and K deficiency tended to suppress waterlogging-induced accumulation of Krebs cycle intermediates in leaves. Furthermore, the natural 15 N/14 N isotope composition (δ15 N) in leaf compounds shows that there was a change in nitrate circulation, with less nitrate influx to leaves under low K availablity combined with waterlogging and more isotopic dilution of lamina nitrates under high K. Our results show that K deficiency and waterlogging effects are not simply additive, reshape respiration as well as nitrogen metabolism and partitioning, and are associated with metabolomic and isotopic biomarkers of potential interest for crop monitoring. |
| Databáze: | OpenAIRE |
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