Visualization of subsurface blood vessels by color Doppler optical coherence tomography in rats: before and after hemostatic therapy
| Autor: | Manish D. Kulkarni, Joseph A. Izatt, Siavash Yazdanfar, Michael V. Sivak, Richard C.K. Wong, Joseph Willis, Jennifer K. Barton, Ashley J. Welch |
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| Rok vydání: | 2002 |
| Předmět: |
Pathology
medicine.medical_specialty Epinephrine genetic structures medicine.medical_treatment Surgical Flaps Rats Sprague-Dawley symbols.namesake Optical coherence tomography Electrocoagulation medicine Animals Vasoconstrictor Agents Radiology Nuclear Medicine and imaging Ultrasonography Doppler Color Tomography Skin Laser Coagulation medicine.diagnostic_test Hemostatic Techniques Vascular disease business.industry Hemostasis Endoscopic Gastroenterology Blood flow medicine.disease Sclerosing Solutions Rats medicine.anatomical_structure Hemostasis symbols Blood Vessels business Doppler effect Laser coagulation Blood Flow Velocity Blood vessel Biomedical engineering |
| Zdroj: | Gastrointestinal Endoscopy. 55:88-95 |
| ISSN: | 0016-5107 |
| Popis: | Background: The ability to visualize subsurface blood vessels and measure flow may be useful in certain experimental and clinical settings. Methods: Color Doppler optical coherence tomography was used to visualize and measure blood flow in subsurface vessels in vivo in a rat skin flap model. Local "hemostatic" interventions (epinephrine or sclerosant injection, heat probe, and laser) were then applied and imaging was repeated. The skin flap was evaluated histologically. Results: Subsurface blood vessels were easily visualized in cross-section, and vessel diameter and bidirectional blood flow velocity were readily measured. Color Doppler optical coherence tomography demonstrated that flow was significantly reduced after epinephrine injection and became undetectable after the other interventions. This correlated with pathologic evidence of vessel damage in all interventions, except for epinephrine injection. Although vessel response was as predicted to most interventions, the response to epinephrine was only temporary, and limited application of heat alone from the heat probe halted flow without visually apparent surface injury. Conclusions: Color Doppler optical coherence tomography provides high-resolution, cross-sectional flow imaging in subsurface blood vessels. Color Doppler optical coherence tomography is potentially a better technique for the study of existing and new hemostatic intervention in the laboratory. Potential future clinical applications include monitoring of the response to hemostatic modalities. |
| Databáze: | OpenAIRE |
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