Facile Fabrication of Tissue-Engineered Constructs Using Nanopatterned Cell Sheets and Magnetic Levitation
Autor: | Nisa Penland, Eunpyo Choi, Deok Ho Kim, Mikael Perla, Jungyul Park |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Fabrication Materials science Cell Culture Techniques Bioengineering Nanotechnology 02 engineering and technology Article Cell Line Myoblasts 03 medical and health sciences Mice Monolayer Animals General Materials Science Nanotopography Electrical and Electronic Engineering Magnetite Nanoparticles Magnetic levitation Cell sheet Acrylamides Tissue Engineering Mechanical Engineering General Chemistry 021001 nanoscience & nanotechnology 030104 developmental biology Magnetic Fields Mechanics of Materials Magnet Levitation Magnets Wettability Magnetic nanoparticles 0210 nano-technology |
Popis: | We report a simple and versatile method for in vitro fabrication of scaffold-free tissue-engineered constructs with predetermined cellular alignment, by combining magnetic cell levitation with thermoresponsive nanofabricated substratum (TNFS) based cell sheet engineering technique. The TNFS based nanotopography provides contact guidance cues for regulation of cellular alignment and enables cell sheet transfer, while magnetic nanoparticles facilitate the magnetic levitation of the cell sheet. The temperature-mediated change in surface wettability of the thermoresponsive poly(N-isopropylacrylamide), PNIPAM, substratum enables the spontaneous detachment of cell monolayers, which can then be easily manipulated through use of a ring or disk shaped magnet. Our developed platform could be readily applicable to production of tissue-engineered constructs containing complex physiological structures for the study of tissue structure-function relationships, drug screening, and regenerative medicine. |
Databáze: | OpenAIRE |
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