Limitations Due to Unstirred Layers in Measuring Channel Response of Excised Membrane Patch Using Rapid Solution Exchange Methods
| Autor: | A Noma, Dayi Qin, Atsuya Yoshida |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Neurons
Potassium Channels Physiology chemistry.chemical_element Rats Inbred Strains General Medicine Anatomy In Vitro Techniques Calcium Electrochemistry Hippocampus Potassium channel Rats Electrophysiology Membrane chemistry Biophysics Animals Hippocampal neuron Patch clamp Ion Channel Gating Intracellular |
| Zdroj: | The Japanese Journal of Physiology. 41:333-339 |
| ISSN: | 0021-521X |
| Popis: | The Ca(2+)-activated K+ channel was activated by a step increase in the intracellular Ca2+ concentration ([Ca2+]i) using the oil-gate concentration jump method in hippocampal neurons of rat. Limitations due to the unstirred volume between the excised inside-out membrane and the tip opening were examined. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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