Reproductive toxicity of polystyrene microplastics: In vivo experimental study on testicular toxicity in mice
| Autor: | Baolian Hou, Fangyi Wang, Tao Liu, Zhiping Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
congenital hereditary and neonatal diseases and abnormalities Environmental Engineering Health Toxicology and Mutagenesis Microplastics 0211 other engineering and technologies 02 engineering and technology 010501 environmental sciences Biology 01 natural sciences Blood cell Andrology Mice Western blot In vivo Testis medicine Environmental Chemistry Animals skin and connective tissue diseases Waste Management and Disposal 0105 earth and related environmental sciences 021110 strategic defence & security studies Mice Inbred ICR medicine.diagnostic_test nutritional and metabolic diseases Interleukin Epididymis Pollution Sperm medicine.anatomical_structure Polystyrenes Reproductive toxicity Spermatogenesis Plastics |
| Zdroj: | Journal of hazardous materials. 405 |
| ISSN: | 1873-3336 |
| Popis: | Microplastics (MPS) are widespread in our environment and have a potential impact on the reproductive development of humans and mammals. In this study, we evaluated the effect of 5 µm polystyrene microplastics(PS-MPS) on spermatogenesis in mice. The damage by PS-MPS to epididymal sperm was studied using blood cell counts. The results showed that the number of viable epididymis sperm after PS-MPS exposure was significantly reduced. Using Duff-Quik staining, we found that the PS-MPS exposure increased the rate of sperm deformity. The testis is an important organ responsible for normal spermatogenesis. HE and TUNEL staining showed atrophy, shedding, and apoptosis of sperm cells at all levels of the testis after exposure to PS-MPS. Western blot and qPCR analysis were used to detect Nrf2/HO-1 and NF-κB. The results showed that after PS-MPS exposure, the expression of the pro-inflammatory molecule NF-κB and that of the inflammatory factors interleukin (IL)-1β and IL-6 increased significantly, whereas that of the anti-inflammatory molecule Nrf2/HO-1 decreased. These results indicate that the abnormal sperm quality in ICR mice caused by PS-MPS exposure is closely related to the Nrf2/HO-1/NF-κB pathway. |
| Databáze: | OpenAIRE |
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