Evaluation of extraction-free RT-qPCR methods for SARS-CoV-2 diagnostics
| Autor: | Patrizia Caligiuri, Bianca Bruzzone, Andrea Orsi, Beatrice Marina Pennati, Alexander Domnich, Vanessa De Pace, Serena Varesano |
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| Rok vydání: | 2021 |
| Předmět: |
2019-20 coronavirus outbreak
Coronavirus disease 2019 (COVID-19) SARS-CoV-2 Brief Report Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Extraction (chemistry) COVID-19 Negativity effect General Medicine Gold standard (test) Biology Sensitivity and Specificity Virology Reverse transcriptase Specimen Handling Real-time polymerase chain reaction COVID-19 Nucleic Acid Testing Humans RNA Viral |
| Zdroj: | Archives of Virology |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | Extraction-based real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) is currently the "gold standard" in SARS-CoV-2 diagnostics. However, some extraction-free RT-qPCR techniques have recently been developed. In this study, we compared the sensitivity of traditional extraction-based, heated extraction-free, and unheated extraction-free RT-qPCR methods for SARS-CoV-2 detection in nasopharyngeal swabs from symptomatic individuals. The unheated extraction-free method showed perfect agreement with the standard extraction-based RT-qPCR. By contrast, the heat-treated technique was associated with an 8.2% false negativity rate. Unheated extraction-free RT-qPCR for the molecular diagnosis of SARS-CoV-2 is a valuable alternative to the traditional extraction-based methods and may accelerate turnaround times by about two hours. |
| Databáze: | OpenAIRE |
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