A Novel Mechanism of Anti–T-Lymphocyte Globulin Mediated by Fractalkine in Renal Ischemia–Reperfusion Injury in Rats
| Autor: | O. Selvi, A. Inal, Y. Gunay, Neşe Yener, Orhun Sinanoglu, Hüseyin Yüce Bircan |
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| Přispěvatelé: | Maltepe Üniversitesi |
| Rok vydání: | 2013 |
| Předmět: |
Male
Chemokine medicine.medical_specialty Time Factors Globulin T-Lymphocytes Lymphocyte medicine.medical_treatment Kidney chemistry.chemical_compound Internal medicine Animals Medicine Warm Ischemia Rats Wistar Saline Kidney transplantation Antilymphocyte Serum Transplantation Creatinine biology Chemokine CX3CL1 urogenital system business.industry Chemotaxis Macrophages Dendritic Cells T lymphocyte medicine.disease Rats Disease Models Animal Endocrinology medicine.anatomical_structure chemistry Cytoprotection Reperfusion Injury Immunology biology.protein Kidney Diseases Surgery business Biomarkers Immunosuppressive Agents |
| Zdroj: | Transplantation Proceedings. 45:2461-2468 |
| ISSN: | 0041-1345 |
| Popis: | WOS: 000323852000069 PubMed ID: 23953563 Background. Ischemia-reperfusion injury (IRI) is among the main challenges in kidney transplantation. It causes delayed graft function and graft loss in long-term follow-up studies. Anti-T lymphocyte globulin (ATG), a common induction immunosuppressive, has been used in kidney transplantation to prevent rejection. Fractalkine (FKN) is among the main chemokines involved in IRI. This study was designed to identify the relationship between ATG and FKN after warm ischemia in rat kidneys. Methods. Rats were divided into three groups: Control, IRI+normal saline(NS) and IRI+ATG. After IRI was initiated, rats received a dose of ATG or NS during surgery as well as two more doses at 24 and 48 hours after surgery. All rats were humanely killed at 72 hours. Results. The concentration of FKN as well as dendritic cells (DC) and macrophages were lower in both peripheral blood and the injured kidney among ATG-treated versus control rats. Additionally cell necrosis, cytoplasmic vacuolization, cast formation, and tubular dilatation were improved among ATG-treated rats. Serum creatinine levels were lower in rats that received ATG. Conclusion. ATG depleted the concentration of FKN, which inhibits migrations of DCs and macrophages into the kidney, and reduces IRI-related pathology. |
| Databáze: | OpenAIRE |
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