Regulation of steroid production in ovarian stromal tissue from 5- to 8-week-old pullets and laying hens
| Autor: | J. M. Levorse, Alan L. Johnson |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Embryology
medicine.medical_specialty Stromal cell 8-Bromo Cyclic Adenosine Monophosphate Biology Adenylyl cyclase chemistry.chemical_compound Endocrinology Internal medicine medicine Animals Sexual Maturation Androstenedione Gonadal Steroid Hormones Calcimycin Cells Cultured Progesterone Estradiol Colforsin Ovary Obstetrics and Gynecology Cytochrome P450 Cell Biology Luteinizing Hormone Transforming Growth Factor alpha In vitro Reproductive Medicine chemistry Theca biology.protein Tetradecanoylphorbol Acetate Female Signal transduction Chickens Hormone |
| Zdroj: | Reproduction. 100:195-202 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/jrf.0.1000195 |
| Popis: | Studies were conducted to evaluate the regulation of steroid production in dispersed cells from ovarian stromal tissue from 5- to 8-week-old-pullets (IM cells) and laying hens (MAT cells). Short-term incubation of IM and MAT cells with ovine (o) LH resulted in a dose-dependent increase in progesterone, androstenedione and oestradiol production; progesterone production was greater in MAT cells than in IM cells (P0.05) in response to 2-200 ng oLH ml-1, whereas androstenedione and oestradiol production was greater in MAT cells following treatment with 20 and 200 ng oLH ml-1 (P0.05). In both cell populations the cyclic adenosine monophosphate (cAMP) analogue, 8-bromo-cAMP (1 and 10 mmol l-1) stimulated progesterone and androstenedione production, whereas oLH (200 ng ml-1) and forskolin (1-10 mumol l-1) promoted cAMP accumulation (P0.05 compared with basal values). However, treatment with the protein kinase C activator, phorbol 12-myristate 13-acetate (PMA), did not alter basal or oLH-stimulated cAMP accumulation or progesterone production in either IM or MAT cells (P0.10). PMA did, however, inhibit agonist-induced androstenedione production (P0.05); co-treatment with the calcium ionophore A23187 potentiated this inhibitory effect. Finally, treatment with transforming growth factor-alpha (TGF-alpha; 1.8-18 pmol l-1) did not affect basal or oLH-stimulated progesterone or androstenedione production by IM cells, MAT cells, theca cells from 6-8 mm follicles or theca cells from the second largest (F2) follicle (P0.10).(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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