Characterization of Bean common mosaic virus and Bean common mosaic necrosis virus isolates in common bean growing areas in Turkey
| Autor: | Ilyas Deligoz, Nazli Dide Kutluk-Yilmaz, Miray Arli-Sokmen |
|---|---|
| Přispěvatelé: | Ondokuz Mayıs Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine BCMV Bean common mosaic virus I gene Plant Science Horticulture Biology 01 natural sciences 03 medical and health sciences Cultivar Allele Gene BCMNV Strain (biology) Nucleic acid sequence food and beverages Virology Sequence identity Bean common mosaic necrosis virus 030104 developmental biology CP pathogroup Agronomy and Crop Science bc-alleles 010606 plant biology & botany |
| Popis: | Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) are well-known legume-infecting potyviruses. The incidences of BCMV and BCMNV infections were determined by ELISA in 367 seed and leaf samples which were collected in 15 common bean-growing provinces of Turkey. Of the samples tested, 67 (18.2 %) occurred to be infected with BCMV, however only 5 (1.4 %) were infected with BCMNV. A total of 45 ELISA-positive samples were selected from single-virus infected ones to determine BCMV and BCMNV pathogenicity groups (PGs) by using a set of bean cultivars that contain different combinations of resistance genes. Some BCMV populations exhibiting unusual pathogenicity were identified. One of them, named TR-180, was found to overcome resistance conferred by bc-1, bc-1 2 , bc-2 and bc-2 2 recessive alleles in common bean and assigned to PG VII. This isolate shared high (99 %) sequence identity with previously identified BCMV RU-1 and RU-1-related strains (RU1-OR-B and RU1-OR-C) according to a BLAST analysis of the nucleotide sequences of RT-PCR amplified products comprising the complete coat protein and 3′ partial NIb regions. The isolates TR-203 and TR-256 produced a distinctive reaction pattern in the dominant I gene-bearing bean cultivars Amanda and Isabella at lower ( |
| Databáze: | OpenAIRE |
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