Effects of Dietary Copper-Methionine on Matrix Metalloproteinase-2 in the Lungs of Cold-Stressed Broilers as an Animal Model for Pulmonary Hypertension
| Autor: | Gwenn Perrot, Amir Hossein Mahdavi, Stéphane Brézillon, Mina Bagheri Varzaneh, Hamid Reza Rahmani, Corinne Perreau, François-Xavier Maquart, R. Jahanian |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Endocrinology Diabetes and Metabolism Hypertension Pulmonary Clinical Biochemistry Matrix metalloproteinase Biochemistry Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Animal science Methionine Ascites medicine Animals RNA Messenger Lung Completely randomized design Dose-Response Relationship Drug business.industry Biochemistry (medical) Broiler Pericardial fluid General Medicine medicine.disease Pulmonary hypertension Disease Models Animal 030104 developmental biology medicine.anatomical_structure chemistry Dietary Supplements Matrix Metalloproteinase 2 medicine.symptom business Chickens 030217 neurology & neurosurgery Copper |
| Zdroj: | Biological trace element research. 172(2) |
| ISSN: | 1559-0720 |
| Popis: | The objective of the present study was to investigate the effects of different levels of copper (as supplemental copper-methionine) on ascites incidence and matrix metalloproteinase-2 (MMP-2) changes in the lungs of cold-stressed broilers. For this purpose, 480 1-day-old Ross 308 broiler chickens were randomly assigned to six treatments. Treatments consisted of two ambient temperatures (thermoneutral and cold stress) each combined with 0, 100, and 200 mg supplemental copper/kg as copper-methionine in a 2 × 3 factorial arrangement in a completely randomized design with four replicates. Ascites was diagnosed based on abdominal and pericardial fluid accumulation at 45 days of age. Fourty-eight broilers were killed at 38 and 45 days of age, and their lungs were collected for biological analysis. Results showed that MMP-2 increased in the lungs of ascitic broilers and that copper-methionine supplementation significantly reduced MMP-2 in cold-stressed broiler chickens. Treatments did not affect tissue inhibitor of metalloproteinase-2 (TIMP-2) at 38 and 45 days of age, and no difference was observed between 100 and 200 mg/kg copper-methionine treatments. In conclusion, copper-methionine at higher than conventional levels of supplementation decreased ascites incidence in low temperature through reduced MMP-2 concentration. Further research is warranted to investigate the effect of copper on MMP-2 concentrations in other tissues with high oxygen demand. |
| Databáze: | OpenAIRE |
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