BRCA1 Down-Regulates Cellular Levels of Reactive Oxygen Species
Autor: | Tapas Saha, Eliot M. Rosen, Jeong Keun Rih |
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Jazyk: | angličtina |
Rok vydání: | 2009 |
Předmět: |
Antioxidant
endocrine system diseases medicine.medical_treatment Biophysics 8-Oxoguanine Down-Regulation Breast Neoplasms Biology medicine.disease_cause Biochemistry Article Flow cytometry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation Structural Biology Cell Line Tumor Genetics medicine Humans RNA Small Interfering skin and connective tissue diseases Molecular Biology REF1 030304 developmental biology chemistry.chemical_classification Nitrotyrosine 0303 health sciences Reactive oxygen species Microscopy Confocal medicine.diagnostic_test Base Sequence BRCA1 Protein Reactive oxygen species (ROS) Cell Biology BRCA1 Flow Cytometry Cell biology Oxidative Stress chemistry 030220 oncology & carcinogenesis Reactive Oxygen Species Oxidative stress Intracellular |
Popis: | Previous studies have shown that the breast cancer suppressor BRCA1 stimulates antioxidant gene expression and protects cells against oxidative stress. To further examine this important function, we tested whether BRCA1 could modulate intracellular levels of reactive oxygen species (ROS). Wild-type BRCA1 (but not a cancer-associated mutant) significantly reduced ROS levels, determined by DCF fluorescence assays by flow cytometry and confocal microscopy. The BRCA1 and REF1 pathways for reduction of ROS levels appear to exhibit cross-talk. BRCA1 also reduced the levels of protein nitration and H2O2-induced oxidative damage to DNA. Thus, BRCA1 may protect cellular macromolecules by reducing intracellular ROS levels. |
Databáze: | OpenAIRE |
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