BRCA1 Down-Regulates Cellular Levels of Reactive Oxygen Species

Autor: Tapas Saha, Eliot M. Rosen, Jeong Keun Rih
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Antioxidant
endocrine system diseases
medicine.medical_treatment
Biophysics
8-Oxoguanine
Down-Regulation
Breast Neoplasms
Biology
medicine.disease_cause
Biochemistry
Article
Flow cytometry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Downregulation and upregulation
Structural Biology
Cell Line
Tumor

Genetics
medicine
Humans
RNA
Small Interfering

skin and connective tissue diseases
Molecular Biology
REF1
030304 developmental biology
chemistry.chemical_classification
Nitrotyrosine
0303 health sciences
Reactive oxygen species
Microscopy
Confocal

medicine.diagnostic_test
Base Sequence
BRCA1 Protein
Reactive oxygen species (ROS)
Cell Biology
BRCA1
Flow Cytometry
Cell biology
Oxidative Stress
chemistry
030220 oncology & carcinogenesis
Reactive Oxygen Species
Oxidative stress
Intracellular
Popis: Previous studies have shown that the breast cancer suppressor BRCA1 stimulates antioxidant gene expression and protects cells against oxidative stress. To further examine this important function, we tested whether BRCA1 could modulate intracellular levels of reactive oxygen species (ROS). Wild-type BRCA1 (but not a cancer-associated mutant) significantly reduced ROS levels, determined by DCF fluorescence assays by flow cytometry and confocal microscopy. The BRCA1 and REF1 pathways for reduction of ROS levels appear to exhibit cross-talk. BRCA1 also reduced the levels of protein nitration and H2O2-induced oxidative damage to DNA. Thus, BRCA1 may protect cellular macromolecules by reducing intracellular ROS levels.
Databáze: OpenAIRE