Identification of mobile colistin resistance genes (mcr-1.1, mcr-5 and mcr-8.1) in Enterobacteriaceae and Alcaligenes faecalis of human and animal origin, Nigeria
| Autor: | Patrick Butaye, Patrick Kelly, A. M. Adamu, Levi M. Mamfe, Emmanuel O. Ngbede, Anwar Kalalah, Salem T. Daniel, Yi Yang, Nicodemus M. Useh, Mohammed I. Adah, Anil Poudel, Folasade Adekanmbi, Alex A. Adikwu, Chengming Wang, Jacob K. P. Kwaga |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Carbapenem Retroelements Swine 030106 microbiology Nigeria Microbial Sensitivity Tests Microbiology 03 medical and health sciences chemistry.chemical_compound Dogs 0302 clinical medicine Enterobacteriaceae Drug Resistance Multiple Bacterial medicine Animals Humans Pharmacology (medical) 030212 general & internal medicine Alcaligenes faecalis biology Colistin General Medicine biology.organism_classification Anti-Bacterial Agents Multiple drug resistance Infectious Diseases chemistry Cattle MCR-1 Alcaligenes MacConkey agar hormones hormone substitutes and hormone antagonists Plasmids medicine.drug |
| Zdroj: | International Journal of Antimicrobial Agents. 56:106108 |
| ISSN: | 0924-8579 |
| DOI: | 10.1016/j.ijantimicag.2020.106108 |
| Popis: | Colistin is a last resort drug used to treat infections caused by multidrug resistant Gram-negative bacteria that have developed resistance to carbapenem. The emergence and rapid dissemination of the nine plasmid-mediated mobile colistin resistance genes (mcr-1 to mcr-9) has led to fear of pan-drug resistant infections occurring worldwide. To date, there is only limited information on colistin resistance in African countries where the drug is widely used in agriculture. In this study from Nigeria, 583 non-duplicate bacterial strains were isolated from 1,119 samples collected from humans, camels, cattle, dogs, pigs and poultry by the use of colistin-supplemented MacConkey agar, and 17.0% of the isolates (99/583) were colistin resistant. PCRs (mcr-1 to mcr-9) and whole genome sequencing (WGS) identified mcr in 21.2% of the colistin-resistant isolates: mcr-1.1 (n=13), mcr-8.1 (n=5), both mcr-1.1 and mcr-8.1 (n=2), and mcr-1.1 and mcr-5 (n=1). Nine of the 21 mcr-positive strains were isolated from human samples, with eight being K. pneumoniae, and six of these human K. pneumoniae had a high colistin MIC of >64 µg/ml. In contrast, nine of the 12 mcr-positive animal isolates were E. coli of which only two had a colistin MIC of >64 µg/ml. Our study is the first to report mcr-1 in Alcaligenes faecalis, and emergence of mcr-5 and mcr-8 in Nigeria. WGS determined that mcr-1 was localized on IncX4 plasmid, and 95.2% of mcr-1 harboring isolates (20/21) transferred colistin resistance successfully in conjugation assay. Our findings highlight the global spread of colistin resistance, and emphasize the urgent need for coordinated global action to combat resistant bacteria. |
| Databáze: | OpenAIRE |
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