The Laetiporus polyketide synthase LpaA produces a series of antifungal polyenes
Autor: | Markus Gressler, Paula Sophie Seibold, Dirk Hoffmeister, Claudius Lenz |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Antifungal Agents 030106 microbiology Multienzyme complexes Microbial Sensitivity Tests Polyenes 01 natural sciences Aspergillus nidulans 03 medical and health sciences chemistry.chemical_compound Biosynthesis Polyketide synthase Drug Discovery Pharmacology Aspergillus biology ATP synthase 010405 organic chemistry Special Feature: Article Aspergillus niger Polyene biology.organism_classification 0104 chemical sciences Fungal physiology chemistry Biochemistry biology.protein Polyporales Polyketide Synthases Laetiporus |
Zdroj: | The Journal of Antibiotics |
ISSN: | 1881-1469 0021-8820 |
Popis: | The conspicuous bright golden to orange-reddish coloration of species of the basidiomycete genus Laetiporus is a hallmark feature of their fruiting bodies, known among mushroom hunters as the “chicken of the woods”. This report describes the identification of an eight-domain mono-modular highly reducing polyketide synthase as sole enzyme necessary for laetiporic acid biosynthesis. Heterologous pathway reconstitution in both Aspergillus nidulans and Aspergillus niger verified that LpaA functions as a multi-chain length polyene synthase, which produces a cocktail of laetiporic acids with a methyl-branched C26–C32 main chain. Laetiporic acids show a marked antifungal activity on Aspergillus protoplasts. Given the multiple products of a single biosynthesis enzyme, our work underscores the diversity-oriented character of basidiomycete natural product biosynthesis. |
Databáze: | OpenAIRE |
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