Introgression of B-genome chromosomes in a doubled haploid population ofBrassica napus ×B. carinata
| Autor: | J C Pires, Z Xiong, Allen G. Good, M. R. Thiagarajah, Z. K. Navabi, Muhammad H. Rahman, Isobel A. P. Parkin |
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| Rok vydání: | 2010 |
| Předmět: |
Genotype
Population Brassica Introgression Haploidy Hybrid Cells Plant disease resistance Genome Chromosomes Plant Botany Genetics education Molecular Biology Crosses Genetic In Situ Hybridization Fluorescence education.field_of_study biology Brassica napus Brassica carinata Chromosome Mapping General Medicine biology.organism_classification Genetics Population Doubled haploidy Hybridization Genetic Microsatellite Genome Plant Biotechnology |
| Zdroj: | Genome. 53:619-629 |
| ISSN: | 1480-3321 0831-2796 |
| Popis: | The Brassica B-genome species possess many valuable agronomic and disease resistance traits. To transfer traits from the B genome of B. carinata into B. napus , an interspecific cross between B. napus and B. carinata was performed and a doubled haploid (DH) population was generated from the BC2S3generation. Successful production of interspecific DH lines as identified using B-genome microsatellite markers is reported. Five percent of DH lines carry either intact B-genome chromosomes or chromosomes that have deletions. All of the DH lines have linkage group J13/B7 in common. This was further confirmed using B. nigra genomic DNA in a fluorescent in situ hybridization assay where the B-genome chromosomes were visualized and distinguished from the A- and C-genome chromosomes. The 60 DH lines were also evaluated for morphological traits in the field for two seasons and were tested for resistance to blackleg, caused by Leptosphaeria maculans , under greenhouse conditions. Variation in the DH population followed a normal distribution for several agronomic traits and response to blackleg. The lines with B-genome chromosomes were significantly different (p < 0.01) from the lines without B-genome chromosomes for both morphological and seed quality traits such as days to flowering, days to maturity, and erucic acid content. |
| Databáze: | OpenAIRE |
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