Inhibition of Atherosclerotic Plaque Development by Oral Administration of α-Glucosyl Hesperidin and Water-Dispersible Hesperetin in Apolipoprotein E Knockout Mice
| Autor: | Ayako Katagi, Tetsuya Kitagawa, Michio Shimabukuro, Masataka Sata, Noriko Sugasawa, Hiroko Takumi, Hirotsugu Kurobe, Chika Nishio, Fumiharu Higashiguchi, Ken-ichi Aihara, Taisuke Nakayama |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Apolipoprotein E Male Antioxidant medicine.medical_treatment Flavonoid Medicine (miscellaneous) Administration Oral 030209 endocrinology & metabolism Pharmacology Diet High-Fat Antioxidants 03 medical and health sciences chemistry.chemical_compound Hesperidin Mice 0302 clinical medicine Apolipoproteins E Glucosides Oral administration medicine Animals chemistry.chemical_classification Mice Knockout 030109 nutrition & dietetics Nutrition and Dietetics Hesperetin food and beverages Plaque Atherosclerotic Aglycone Cholesterol chemistry Knockout mouse |
| Zdroj: | Journal of the American College of Nutrition. 38(1) |
| ISSN: | 1541-1087 |
| Popis: | Hesperidin, an abundant flavonoid in citrus fruit, and its aglycone, hesperetin, have been reported to possess various physiological activities, including antioxidant, anti-inflammatory, hypolipidemic, and antihypertensive activities. In this study, we investigated whether α-glucosyl hesperidin and water-dispersible hesperetin have protective effects on atherosclerotic progression in apolipoprotein E knockout (Apo-E KO) mice.Ten-week-old male Apo-E KO mice were randomly assigned a regular high-fat diet, a high-fat diet with 0.5% α-glucosyl hesperidin, or a high-fat diet with 0.1% water-dispersible hesperetin for 12 weeks. Measurement of plasma total cholesterol levels, histological staining of aortic root, and immunohistochemistry for macrophages were performed to evaluate atherosclerotic plaque formation. Vascular reactivity of mouse aortic rings was also measured.Both α-glucosyl hesperidin and water-dispersible hesperetin reduced plasma total cholesterol level. They also reduced plaque formation area, adipose deposition, and macrophage infiltration into atherosclerotic lesion. Vascular-endothelium-dependent relaxation in response to acetylcholine was improved in both experimental diet groups compared to the high-fat diet group.Our study suggests that both α-glucosyl hesperidin and water-dispersible hesperetin exert protective effects on atherosclerotic progression in Apo-E KO mice because they exhibit hypolipidemic activity, reduce inflammation through macrophages, and prevent endothelial dysfunction. |
| Databáze: | OpenAIRE |
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