Core genome multi-locus sequence typing as an essential tool in a high-cost livestock-associated meticillin-resistant Staphylococcus aureus CC398 hospital outbreak
| Autor: | Henrik Westh, Hans Jørn Kolmos, Michael Kemp, M.L. Slott Jensen, H. Lundgaard, Anette Holm, Annette Toft, Heidi Gumpert, M. Nielsine Skov, H. Pries Kristiansen |
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| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Methicillin-Resistant Staphylococcus aureus medicine.medical_specialty Staphylococcus aureus Livestock Livestock-associated MRSA Denmark Locus (genetics) Single-nucleotide polymorphism 030501 epidemiology medicine.disease_cause Genome Animal Diseases Disease Outbreaks 03 medical and health sciences MRSA CC398 Internal medicine Medicine SNP Animals Humans Typing Whole genome sequencing 0303 health sciences Cross Infection Whole Genome Sequencing 030306 microbiology business.industry Outbreak General Medicine Health Care Costs Staphylococcal Infections Hospital outbreak Infectious Diseases Economic costs cgMLST 0305 other medical science business |
| Zdroj: | Slott Jensen, M L, Nielsine Skov, M, Pries Kristiansen, H, Toft, A, Lundgaard, H, Gumpert, H, Westh, H, Holm, A, Kolmos, H J & Kemp, M 2020, ' Core genome multi-locus sequence typing as an essential tool in a high-cost livestock-associated meticillin-resistant Staphylococcus aureus CC398 hospital outbreak ', Journal of Hospital Infection, vol. 104, no. 4, pp. 574-581 . https://doi.org/10.1016/j.jhin.2019.12.009 |
| ISSN: | 1532-2939 |
| Popis: | Summary Background Livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) clonal complex (CC) 398 may be transmitted and cause morbidity and mortality in hospitals. The economic cost of stopping hospital transmission of LA-MRSA CC398 is poorly described. Early detection of transmission may limit the extent of the intervention. Aim To evaluate core genome multi-locus sequence typing (cgMLST) for detecting transmission chains and to estimate the costs for interventions to prevent further spread after discovery of hospital transmission of LA-MRSA CC398. Methods Five patients were involved in two episodes of transmission of LA-MRSA CC398 in a hospital. Standard interventions including MRSA screening of patients and healthcare workers were initiated. Whole genome sequences of the five isolates and 17 epidemiologically unrelated MRSA CC398 isolates from other hospitalized patients were analysed by single nucleotide polymorphism (SNP) comparisons and cgMLST. The economic costs of constraining transmission were calculated from relevant sources. Findings The five isolates suspected to be involved in hospital transmission clustered with ≤2 SNPs in the draft genome sequences with some distance to other isolates. cgMLST allocated the five isolates to the same type, which was different from all but two of the sporadic isolates. Furthermore, cgMLST separated the five transmission isolates from all other isolates. The economic costs of the outbreak interventions exceeded €11,000 per patient. Conclusion LA-MRSA CC398 is transmittable in hospitals, and intervention against transmission may reach considerable costs. cgMLST is useful in surveillance of hospital transmission of LA-MRSA. |
| Databáze: | OpenAIRE |
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