Expression of M-cadherin protein in myogenic cells during prenatal mouse development and differentiation of embryonic stem cells in culture
| Autor: | Max Rotter, Jürgen Rohwedel, Olaf Rose, Sigrid Reinhardt, Anna M. Wobus, Michael Bachmann, Matthias Cramer, Anna Starzinski-Powitz |
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| Rok vydání: | 1994 |
| Předmět: |
Cell Adhesion Molecules
Neuronal Recombinant Fusion Proteins Molecular Sequence Data Morphogenesis Fluorescent Antibody Technique Gestational Age Biology Embryonic and Fetal Development Mice Laminin Pregnancy Myocyte Animals Amino Acid Sequence RNA Messenger Muscle Skeletal Cells Cultured DNA Primers Mice Inbred BALB C Base Sequence Cadherin Cell adhesion molecule Stem Cells Cell Membrane Gene Expression Regulation Developmental Cadherins Embryonic stem cell Molecular biology Cell culture biology.protein Desmin Female Developmental Biology |
| Zdroj: | Developmental dynamics : an official publication of the American Association of Anatomists. 201(3) |
| ISSN: | 1058-8388 |
| Popis: | Molecules regulating morphogenesis by cell-cell interactions are the cadherins, a class of calcium-dependent adhesion molecules. One of its members, M-cadherin, has been isolated from a myoblast cell line (Donalies et al. [1991] Proc. Natl. Acad. Sci. U.S.A. 88:8024—8028). In mouse development, expression of M-cadherin mRNA first appears at day 8.5 of gestation (E8.5) in somites and has been postulated to be down-regulated in developing muscle masses (Moore and Walsh [1993] Development 117:1409—1420). Affinity-purified polyclonal M-cadherin antibodies, detecting a protein of approximately 120 kDa, were used to study the cell expression pattern of M-cadherin protein. It was first visualized in somites at E10 1/3 and could be confined to desmin positive, myotomal cells. At all subsequent prenatal stages, M-cadherin was only found in myogenic cells of somitic origin. The detection of the protein at E10 1/3 suggests a translational delay of M-cadherin mRNA of 1 to 2 days (E8.5 vs. E10 1/3). This was further supported by the finding that during differentiation of ES cell line BLC6 into skeletal muscle cells in culture, expression of M-cadherin mRNA can be detected 2 days prior to M-cadherin protein. During prenatal development, the pattern of M-cadherin expression changes: In E10 1/3 embryos and also in myotomal cells of later stages, M-cadherin is evenly distributed on the cell surface. In developing muscle masses (tested at E16 to E18), however, M-cadherin protein becomes clustered most likely at sites of cell-cell contact as indicated by double-labelling experiments: M-cadherin-staining is the positive image of laminin negative areas excluding the presence of a basal lamina at M-cadherin positive sites. Furthermore, M-cadherin is coexpressed with the neuronal cell adhesion molecule N-CAM which has been shown to mediate cell-cell contact in myogenic cells. In summary, our results are in line with the idea that M-cadherin might play a central role in myogenic morphogenesis. © 1994 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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