Hepatobiliary transporter expression in percutaneous liver biopsies of patients with cholestatic liver diseases
| Autor: | Kurt Zatloukal, Michael Trauner, Gernot Zollner, Peter Ferenci, Rudolf Stauber, Peter Fickert, Lukas Kenner, Conny Stumptner, Guenter J. Krejs, Andrea Fuchsbichler, Helmut Denk, Rainer Zenz |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Anions Male medicine.medical_specialty ATP Binding Cassette Transporter Subfamily B Biopsy Fluorescent Antibody Technique Alcoholic hepatitis ATP-binding cassette transporter Biology Bile Acids and Salts Primary biliary cirrhosis Cholestasis Reference Values Internal medicine medicine Humans ATP Binding Cassette Transporter Subfamily B Member 1 RNA Messenger Adenosine Triphosphatases Hepatology Multidrug resistance-associated protein 2 Transporter Middle Aged Jaundice medicine.disease Endocrinology Liver ATP-Binding Cassette Transporters Female Bile Ducts medicine.symptom Carrier Proteins Immunostaining |
| Zdroj: | Hepatology. 33:633-646 |
| ISSN: | 0270-9139 |
| DOI: | 10.1053/jhep.2001.22646 |
| Popis: | Reduced hepatobiliary transporter expression could explain impaired hepatic uptake and excretion of bile salts and other biliary constituents resulting in cholestasis and jaundice. Because little is known about alterations of hepatobiliary transport systems in human cholestatic liver diseases, it was the aim of this study to investigate such potential changes. Hepatic mRNA levels in hepatobiliary transport systems for bile salts (NTCP, BSEP), organic anions (OATP2, MRP2, MRP3), organic cations (MDR1), phospholipids (MDR3), and aminophospholipids (FIC1) were determined in 37 human liver biopsies and control livers by competitive reverse-transcription polymerase chain reaction (RT-PCR). Transporter tissue distribution was investigated by immunofluorescence microscopy. In patients with inflammation-induced icteric cholestasis (mainly cholestatic alcoholic hepatitis), mRNA levels of NTCP, OATP2, and BSEP were reduced by 41% (P |
| Databáze: | OpenAIRE |
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