Intestinal epithelial organoids fuse to form self-organizing tubes in floating collagen gels
Autor: | Norman Sachs, Peter J. Peters, Pekka Kujala, Yoshiyuki Tsukamoto, Hans Clevers |
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Přispěvatelé: | RS: M4I - Nanoscopy, Institute of Nanoscopy (IoN), Hubrecht Institute for Developmental Biology and Stem Cell Research |
Rok vydání: | 2017 |
Předmět: |
Organoid
0301 basic medicine FIBROBLASTS Cell type Mouse Cellular differentiation Biology Research Support Cell Fusion Tissue Culture Techniques CULTURE Mice 03 medical and health sciences Matrigel Collagen gel 0302 clinical medicine Intestinal mucosa Tube Mini-gut CONTRACTION Cell polarity Journal Article Animals Intestinal Mucosa Non-U.S. Gov't Molecular Biology GENE-EXPRESSION Tube formation CRYPT Stem Cells Research Support Non-U.S. Gov't Cell Differentiation IN-VITRO Anatomy Rats Cell biology Organoids STEM-CELL NICHE DIFFERENTIATION 030104 developmental biology 030220 oncology & carcinogenesis MORPHOGENESIS Collagen Stem cell Gels Developmental Biology |
Zdroj: | Development, 144(6), 1107-1112. Company of Biologists Ltd Development (Cambridge), 144(6), 1107-1112. Company of Biologists Ltd Development (Cambridge, England), 144(6), 1107. Company of Biologists Ltd |
ISSN: | 1477-9129 0950-1991 |
Popis: | Multiple recent examples highlight how stem cells can self-organize in vitro to establish organoids that closely resemble their in vivo counterparts. Single Lgr5+ mouse intestinal stem cells can be cultured under defined conditions forming ever-expanding epithelial organoids that retain cell polarization, cell type diversity and anatomical organization of the in vivo epithelium. Although exhibiting a remarkable level of self-organization, the so called ‘mini-guts’ have a closed cystic structure of microscopic size. Here, we describe a simple protocol to generate macroscopic intestinal tubes from small cystic organoids. Embedding proliferating organoids within a contracting floating collagen gel allows them to align and fuse to generate macroscopic hollow structures (‘tubes’) that are lined with a simple epithelium containing all major cell types (including functional stem cells) of the small intestine. Cells lining the central contiguous lumen closely resemble the epithelial cells on luminal villi in vivo, whereas buds that protrude from the main tube into the surrounding matrix closely resemble crypts. Thus, the remarkable self-organizing properties of Lgr5+ stem cells extend beyond the level of the microscopic cystic organoid to the next, macroscopic, level of tube formation. |
Databáze: | OpenAIRE |
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