SAF-A forms a complex with BRG1 and both components are required for RNA polymerase II mediated transcription
| Autor: | Rikard Runnberg, Tomas Simonsson, Jessica Ryme, Stina Simonsson, Dzeneta Vizlin-Hodzic |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Transcription
Genetic DNA transcription lcsh:Medicine RNA polymerase II Heterogeneous-Nuclear Ribonucleoprotein U Models Biological Biochemistry Cell Line Molecular Genetics Mice Molecular cell biology RNA interference Nucleic Acids RNA polymerase I Genetics Animals Humans Gene Regulation RNA Messenger lcsh:Science RNA polymerase II holoenzyme Biology Embryonic Stem Cells Multidisciplinary Microscopy Confocal biology General transcription factor Chromosome Biology fungi lcsh:R DNA Helicases Nuclear Proteins Cell Differentiation DNA-Directed RNA Polymerases Molecular biology DNA-Binding Proteins biology.protein Transcription factor II F lcsh:Q Transcription factor II E RNA Polymerase II Gene expression Transcription factor II D Transcription factor II B Protein Binding Transcription Factors Research Article Developmental Biology |
| Zdroj: | PLoS ONE, Vol 6, Iss 12, p e28049 (2011) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | BACKGROUND: Scaffold attachment factor A (SAF-A) participates in the regulation of gene expression by organizing chromatin into transcriptionally active domains and by interacting directly with RNA polymerase II. METHODOLOGY: Here we use co-localization, co-immunoprecipitation (co-IP) and in situ proximity ligation assay (PLA) to identify Brahma Related Gene 1 (BRG1), the ATP-driven motor of the human SWI-SNF chromatin remodeling complex, as another SAF-A interaction partner in mouse embryonic stem (mES) cells. We also employ RNA interference to investigate functional aspects of the SAF-A/BRG1 interaction. PRINCIPAL FINDINGS: We find that endogenous SAF-A protein interacts with endogenous BRG1 protein in mES cells, and that the interaction does not solely depend on the presence of mRNA. Moreover the interaction remains intact when cells are induced to differentiate. Functional analyses reveal that dual depletion of SAF-A and BRG1 abolishes global transcription by RNA polymerase II, while the nucleolar RNA polymerase I transcription machinery remains unaffected. CONCLUSIONS: We demonstrate that SAF-A interacts with BRG1 and that both components are required for RNA Polymerase II Mediated Transcription. |
| Databáze: | OpenAIRE |
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