Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane zone
| Autor: | Susan J. Nishio, Philip L. Sannes, Gregory L. Baker, Laura S. Van Winkle, Michael J. Evans, Charles G. Plopper, April E. Murphy, Michelle V. Fanucchi |
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| Rok vydání: | 2002 |
| Předmět: |
Pulmonary and Respiratory Medicine
Male medicine.medical_specialty Aging Physiology Basic fibroblast growth factor Perlecan Fibroblast growth factor Basement Membrane chemistry.chemical_compound Basement membrane zone Physiology (medical) Internal medicine medicine Animals Tissue Distribution Receptor Fibroblast Growth Factor Type 1 Basement membrane biology Fibroblast growth factor receptor 1 Receptor Protein-Tyrosine Kinases Cell Biology Immunohistochemistry Macaca mulatta Receptors Fibroblast Growth Factor Cell biology Trachea medicine.anatomical_structure Endocrinology chemistry Animals Newborn biology.protein Fibroblast Growth Factor 2 Thickening Collagen Heparan Sulfate Proteoglycans Respiratory tract |
| Zdroj: | American journal of physiology. Lung cellular and molecular physiology. 283(6) |
| ISSN: | 1040-0605 |
| Popis: | Thickening of the basement membrane zone (BMZ) is a characteristic of several airway diseases; however, very little is known about how this process occurs. The purpose of this study was to define development of the BMZ in the trachea of growing rhesus monkeys at 1, 2, 3, and 6 mo of age. We measured immunoreactivity of collagen types I, III, and V to detect structural changes in the developing BMZ. To detect more dynamic, functional components of the epithelial-mesenchymal trophic unit, we evaluated the distribution of perlecan, fibroblast growth factor-2 (FGF-2), and fibroblast growth factor receptor-1 (FGFR-1). One-month-old monkeys had a mean collagen BMZ width of 1.5 ± 0.7 μm that increased to 4.4 ± 0.4 μm in 6-mo-old monkeys. Perlecan was localized in the BMZ of the epithelium at all ages. FGF-2 was strongly expressed in basal cells at 1–3 mo. At 6 mo, FGF-2 was expressed throughout the BMZ and weakly in basal cells. FGFR-1 immunoreactivity was expressed by basal cells and cilia and weakly in the nuclei of columnar cells at all time points. These data indicate that development of the BMZ is a postnatal event in the rhesus monkey that involves FGF-2. |
| Databáze: | OpenAIRE |
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