Impact of the Brown-Midrib bm5 Mutation on Maize Lignins
| Autor: | Laurent Cézard, Dominique Denoue, Valérie Méchin, Frédéric Legée, Aurélia Laluc, Yves Barrière, Catherine Lapierre |
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| Přispěvatelé: | Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Unité de recherche Génétique et Amélioration des Plantes Fourragères (UGAPF), Institut National de la Recherche Agronomique (INRA), ANR Biomass for the Future program [BFF 2012-2019], ANR MAGIC program, UR 0889 Unité Génétique et Amélioration des Plantes Fourragères, Institut National de la Recherche Agronomique (INRA)-Génétique et amélioration des plantes (G.A.P.)-Unité Génétique et Amélioration des Plantes Fourragères (UGAPF) |
| Rok vydání: | 2014 |
| Předmět: |
p-coumaric acid
Coumaric Acids [SDV]Life Sciences [q-bio] Mutant medicine.disease_cause Lignin Zea mays p-Coumaric acid brown-midrib lignin PATHWAY Ferulic acid Cell wall TRANSFERASE chemistry.chemical_compound Zea mays L MOLECULAR-BASIS BRACHYPODIUM-DISTACHYON LIGNIFICATION medicine Propionates Plant Proteins 2. Zero hunger Mutation IDENTIFICATION fungi Acetal food and beverages ACINCORPORATION General Chemistry DEFICIENCY MUTANTS chemistry Biochemistry Seeds DEGRADABILITY General Agricultural and Biological Sciences thioacidolysis ferulic acid |
| Zdroj: | Journal of Agricultural and Food Chemistry Journal of Agricultural and Food Chemistry, American Chemical Society, 2014, 62 (22), pp.5102-5107. ⟨10.1021/jf5019998⟩ |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf5019998 |
| Popis: | We have investigated the impact of the brown-midrib bm5 mutation on lignins and on p-coumaric acid and ferulic acid ester-linked to maize (Zea mays L.) cell walls. Lignified stalks or plant aerial parts (without ears) collected at grain maturity were studied in three genetic backgrounds. Relative to the control, bm5 mutants displayed lower levels of lignins and of p-coumarate esters but increased levels of ferulate esters. Thioacidolysis revealed that bm5 lignins display an increased frequency of free-phenolic guaiacyl units. More importantly, thioacidolysis provided unusual amounts of 1,2,2-trithioethyl ethylguaiacol, a marker compound diagnostic for the incorporation of free ferulic acid into lignins by bis 8-O-4 cross-coupling. As the resulting acetal bonding pattern is a chemically labile branch point introduced in maize lignins by the bm5 mutation, this alteration is prone to facilitate the delignification pretreatments used in the cellulose-to-ethanol process. |
| Databáze: | OpenAIRE |
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