Screening of KRAS Mutation in Pre- and Post-Surgery Serum of Patients Suffering from Colon Cancer by COLD-PCR HRM
| Autor: | Pilar Jiménez-Arriscado, Elena Trujillo-Arribas, Patrocinio Molinero, Juan M. Guerrero, Fernando de la Portilla, Hada C. Macher, Amalia Rubio |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology medicine.medical_specialty Colorectal cancer medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound Internal medicine Medicine Genomic signature Pre and post COLD-PCR business.industry KRAS mutation medicine.disease cf-DNA 030104 developmental biology chemistry Nucleic acid Cancer research KRAS business Kras mutation DNA |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Advances in Experimental Medicine and Biology ISBN: 9783319420424 |
| Popis: | Gahan P., Fleischhacker M., Schmidt B. (eds). Genomic characterization of cell-free circulating tumour DNA (ctDNA) may offer an opportunity to assess clonal dynamics throughout the course of a patient’s illness. The existence of KRAS driver mutations in colon cancer patients is determinant to decide their treatment and to predict their outcome. DNA is extracted automatically from 400 μL of serum using the MagNa Pure Compact with the Nucleic Acid Isolation Kit I. DNA amplification, COLD-PCR and HRM were performed in the same run in the Light Cycler 480. We found three different situations: pre- and post-surgical samples grouped with the negative control, pre-surgical samples appear to group with the positive control and the post-surgical samples appear to group with the negative control and finally both samples, pre- and post-surgical ones, appear to be grouped with the positive control. COLD-PCR HRM is a cost-effective way for screening one of the most common driver mutations to predict the worst prognosis in colorectal cancer. |
| Databáze: | OpenAIRE |
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