Mouse embryonic fibroblasts exhibit extensive developmental and phenotypic diversity
| Autor: | Prabhat K. Singhal, Slim Sassi, Dai Fukumura, Rakesh K. Jain, Brian Seed, Stefan Halvorsen, Lan Lan, Patrick Au |
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| Rok vydání: | 2015 |
| Předmět: |
Vascular Endothelial Growth Factor A
0301 basic medicine Cell type Surface phenotype Green Fluorescent Proteins Gfp reporter Cell Mice Transgenic Cell Separation Biology Cell Line Mice 03 medical and health sciences medicine Animals Multiparameter flow cytometry Fibroblast Multidisciplinary Biological Sciences Fibroblasts Embryo Mammalian Flow Cytometry Phenotype Molecular biology Embryonic stem cell 030104 developmental biology medicine.anatomical_structure Single-Cell Analysis Pericytes Transcriptome |
| Zdroj: | Proceedings of the National Academy of Sciences. 113:122-127 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Analysis of embryonic fibroblasts from GFP reporter mice indicates that the fibroblast cell type harbors a large collection of developmentally and phenotypically heterogeneous subtypes. Some of these cells exhibit multipotency, whereas others do not. Multiparameter flow cytometry analysis shows that a large number of distinct populations of fibroblast-like cells can be found in cultures initiated from different embryonic organs, and cells sorted according to their surface phenotype typically retain their characteristics on continued propagation in culture. Similarly, surface phenotypes of individual cloned fibroblast-like cells exhibit significant variation. The fibroblast cell class appears to contain a very large number of denumerable subtypes. |
| Databáze: | OpenAIRE |
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