Broadening of DNA replication origin usage during metazoan cell differentiation

Autor: Olivier Gandrillon, Sébastien Dazy, Marie-Noëlle Prioleau, Olivier Hyrien
Přispěvatelé: Centre de génétique et de physiologie moléculaire et cellulaire (CGPhiMC), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Régulation de l'expression génétique (REG), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris)-École normale supérieure - Paris (ENS Paris)-Centre National de la Recherche Scientifique (CNRS), Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Association pour la Recherche sur le Cancer, Association Française des Myopathies, Ligue contre le Cancer, Région Rhone-Alpes, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Duponchelle, Martine
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Cellular differentiation
Gene Expression
MESH: DNA Replication
Review Article
Biochemistry
Histones
0302 clinical medicine
Transcription (biology)
MESH: Replication Origin
MESH: Erythroid Progenitor Cells
MESH: Animals
MESH: Histones
MESH: Chromatin Immunoprecipitation
Erythroid Precursor Cells
0303 health sciences
histone acetylation
Acetylation
Cell Differentiation
differentiation
Globins
Chromatin
Histone
MESH: Acetylation
DNA replication origins
MESH: Cell Differentiation
DNA Replication
Chromatin Immunoprecipitation
MESH: Locus Control Region
MESH: Gene Expression
Scientific Report
Replication Origin
erythroid progenitors
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
MESH: Chromatin
03 medical and health sciences
Histone H3
Erythroid Cells
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
[SDV.BBM] Life Sciences [q-bio]/Biochemistry
Molecular Biology
MESH: Globins
Genetics
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Molecular Biology
Gene
030304 developmental biology
MESH: Erythroid Cells
Locus Control Region
Molecular biology
DNA replication origin
biology.protein
Origin recognition complex
chromatin
030217 neurology & neurosurgery
Zdroj: EMBO Reports
EMBO Reports, EMBO Press, 2006, 8, pp.806-811. ⟨10.1038/sj.embor.7400736⟩
EMBO Reports, EMBO Press, 2006, 8, pp.806-811
EMBO Reports, EMBO Press, 2006, 7 (8), pp.806-11. ⟨10.1038/sj.embor.7400736⟩
ISSN: 1469-221X
1469-3178
DOI: 10.1038/sj.embor.7400736⟩
Popis: We have examined whether replication of the chicken beta-globin locus changes during differentiation of primary erythroid progenitors into erythrocytes. In undifferentiated progenitors, four principal initiation sites and a replication fork pausing region (RFP) were observed. Forty-eight hours after induction of differentiation, the principal sites were maintained, even in the activated beta(A)-globin gene, some minor sites were enhanced, three new sites appeared and the RFP disappeared. One of the activated origins showed increased histone H3 K9K14 diacetylation, but the others did not. These results demonstrate a broadening of DNA replication origin usage during differentiation of untransformed metazoan cells and indicate that histone H3 diacetylation, other histone modifications so far reported and transcription are not crucial determinants of origin selection in this system.
Databáze: OpenAIRE
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