Embryonic muscle development in Rainbow trout (Oncorhynchus mykiss) : a scanning electron microscopy and immunohistological study
| Autor: | Benoit Fauconneau, Julien Bobe, Sylvie Andre |
|---|---|
| Přispěvatelé: | Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Embryo
Nonmammalian [SDV]Life Sciences [q-bio] Muscle Fibers Skeletal Biology Sarcomere Notochord Myosin medicine Myocyte Animals [INFO]Computer Science [cs] SALMONIDAE POISSON Muscle Skeletal ComputingMilieux_MISCELLANEOUS Myogenesis Embryogenesis General Medicine Anatomy Immunohistochemistry Cell biology Somite medicine.anatomical_structure Oncorhynchus mykiss Microscopy Electron Scanning Animal Science and Zoology Myofibril |
| Zdroj: | Journal of Experimental Zoology Journal of Experimental Zoology, Wiley, 2000, 286, pp.379-389 |
| ISSN: | 0022-104X 1097-010X |
| Popis: | Embryonic muscle development was studied in rainbow trout (Oncorhynchus mykiss) at low and high temperature using scanning electron microscopy (SEM) and immunohistology. Somite development was described starting at stage 16 (Vernier JM. 1969. Ann Embryol Morphogen 4:495–520) for both temperatures, with special interest in their shape and size. Muscle differentiation, associated with somite growth, is characterized by a larger increase in height compared to width and by acquisition of a chevron shape. Thin structures such as striation, sarcomeres, and myofibrils within muscle cells and myotubes were observed starting at the eyed stage (stage 24). Immunohistological analyses showed appearance of embryonic fast myosin at stage 20 in the deep part of the somite. The area where myosin was expressed extended in the somite throughout embryonic development and the presence of myosin was observed in the entire somite at hatching (stage 30). Slow myosin was expressed in a monolayer of superficial cells at the eyed stage and during the entire embryonic development. Then it was expressed in a few layers of cells located in the red muscle area. These results suggest that muscle differentiation, characterized by myosin expression, is engaged at stage 20. Myogenesis starts in the deep part of the somite, near the notochord and progresses laterally to cover the complete somite at hatching when the somite is composed of muscle fibres exhibiting a high degree of maturity. No significant difference was observed in terms of muscular development between low- and high-temperature conditions. J. Exp. Zool. 286:379–389, 2000. © 2000 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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