Figure S1 from Imaging of Cancer γ-Secretase Activity Using an Inhibitor-Based PET Probe

Autor: Yue-Ming Li, Naga Vara Kishore Pillarsetty, Jason S. Lewis, Lukas M. Carter, Blesida Punzalan, Kelly E. Henry, Georgia Frost, George P. Liao, Thomas Li, Xianzhong Wu, Veronica L. Nagle, Teja Kalidindi, Pengju Nie
Rok vydání: 2023
Popis: Supplementary Figure 1. The radiolabeling product of [124I]PN67 was confirmed by co-injecting cold compound 2 (100uM in 100ul DMSO/H2O/MeCN) with the radioactive fraction (50uCi in 100ul H2O/MeCN) onto HPLC. (A) UV 235/254nm wavelength channels were used to detect absorption of cold PN67. Retention time (RT): 8.17min; (B) The radioactivity was detected by a bioscan flow-count detector. Retention time (RT): 8.28min. Radiochemical purity (>99%) was calculated based on integration of area under curve. The bioscan peak is slightly delayed compared with UV signal because of instrument setting.
Databáze: OpenAIRE