A mouse model of Waardenburg syndrome type IV resulting from an ENU-induced mutation in endothelin 3
| Autor: | Dan Goldowitz, David R. Beier, William J. Pavan, Jody L. Cockroft, Ivana Matera, Jennifer L. Moran |
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| Rok vydání: | 2007 |
| Předmět: |
Candidate gene
Genetic Linkage Clinical Biochemistry Mutant Plant Science Biology Arginine Mice Genetic linkage Animals Humans Point Mutation Histidine Waardenburg Syndrome Allele Transversion education Genetics education.field_of_study Endothelin-3 Pigmentation Point mutation Chromosome Mapping Cell Biology Molecular biology Penetrance Endothelin 3 Disease Models Animal Gene Expression Regulation Ethylnitrosourea Mutation Agronomy and Crop Science Developmental Biology |
| Zdroj: | Pigment cell research. 20(3) |
| ISSN: | 0893-5785 |
| Popis: | A line of mutant mice (114-CH19) exhibiting white spotting and preweaning lethality was identified during an N-ethyl-N-nitrosourea (ENU) mutagenesis screen. The trait segregated as a semidominant bellyspot with reduced penetrance. Homozygous mutant mice showed preweaning lethality, and exhibited white spotting over the majority of the body surface, with pigmented patches remaining around the pinnae, eyes and tail. Linkage analysis localized 114-CH19 on mouse chromosome 2, suggesting endothelin 3 (Edn3) as a candidate gene. Sequence analysis of Edn3 identified a G > A transversion that encodes an arginine to histidine substitution (R96H). This mutation is predicted to disrupt furin-mediated proteolytic cleavage of pro-endothelin that is necessary to form biologically active EDN3. This mutation is novel among human and mouse EDN3 mutants, is the first reported EDN3 ENU mutant, and is the second reported EDN3 point mutation. This study demonstrates the power of using ENU mutagenesis screens to generate new animal models of human disease, and expands the spectrum of EDN3 mutant alleles. |
| Databáze: | OpenAIRE |
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