| Autor: |
Melia Magnen, Ran You, Arjun Rao, Ryan Davis, Lauren Rodriguez, Camille Simoneau, Lisiena Hysenaj, Kenneth Hu, Christina Love, Prescott Woodruff, David Erle, Carolyn Hendrickson, Carolyn Calfee, Michael Matthay, Jeroen Roose, Anita Sil, Melanie Ott, Charles Langelier, Matthew Krummel, Mark Looney |
| Rok vydání: |
2022 |
| Popis: |
In the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic1, considerable focus has been placed on a model of viral entry into host epithelial populations, with a separate focus upon the responding immune system dysfunction that exacerbates or causes disease. We developed a precision-cut lung slice model2,3 to investigate very early host-viral pathogenesis and found that SARS-CoV-2 had a rapid and specific tropism for myeloid populations in the human lung. Infection of alveolar macrophages was partially dependent upon their expression of ACE2, and the infections were productive for amplifying virus, both findings which were in contrast with their neutralization of another pandemic virus, Influenza A virus (IAV). Compared to IAV, SARS-CoV-2 was extremely poor at inducing interferon-stimulated genes in infected myeloid cells, providing a window of opportunity for modest titers to amplify within these cells. Endotracheal aspirate samples from humans with the acute respiratory distress syndrome (ARDS) from COVID-19 confirmed the lung slice findings, revealing a persistent myeloid depot. In the early phase of SARS-CoV-2 infection, myeloid cells may provide a safe harbor for the virus with minimal immune stimulatory cues being generated, resulting in effective viral colonization and quenching of the immune system. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
