Distinct regulatory mechanisms control integrin adhesive processes during tissue morphogenesis
| Autor: | Mary Pines, Guy Tanentzapf, Michael J. Fairchild |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Embryo
Nonmammalian Integrin beta Chains DNA Mutational Analysis Molecular Sequence Data Integrin Morphogenesis Penetrance Biology Models Biological Animals Genetically Modified Extracellular matrix Cell Adhesion Animals Amino Acid Sequence Cell adhesion Cytoskeleton Gene Expression Regulation Developmental Adhesion Phenotype Cell biology biology.protein Drosophila Female Intracellular Protein Binding Developmental Biology |
| Zdroj: | Developmental Dynamics. 240:36-51 |
| ISSN: | 1058-8388 |
| DOI: | 10.1002/dvdy.22488 |
| Popis: | Cell adhesion must be precisely regulated to enable both dynamic morphogenetic processes and the subsequent transition to stable tissue maintenance. Integrins link the intracellular cytoskeleton and extracellular matrix, relaying bidirectional signals across the plasma membrane. In vitro studies have demonstrated that multiple mechanisms control integrin-mediated adhesion; however, their roles during development are poorly understood. We used mutations that activate or deactivate specific functions of vertebrate β-integrins in vitro to investigate how perturbing Drosophila βPS-integrin regulation in developing embryos regulation affects tissue morphogenesis and maintenance. We found that morphogenetic processes use various β-integrin regulatory mechanisms to differing degrees and that conformational changes associated with outside-in activation are essential for developmental integrin functions. Long-term adhesion is also sensitive to integrin dysregulation, suggesting integrins must be continuously regulated to support stable tissue maintenance. Altogether, in vivo phenotypic analyses allowed us to identify the importance of various β-integrin regulatory mechanisms during different morphogenetic processes. Developmental Dynamics 240:36–51, 2011. © 2010 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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