Calcium neuroimaging in behaving zebrafish larvae using a turn-key light field camera
| Autor: | Gil G. Westmeyer, Michele Cappetta, Carlos Cruz Perez, Antonella Lauri, Arne Erdmann, Panagiotis Symvoulidis |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Computer science
Biomedical Engineering Neuroimaging Biomaterials Calcium imaging Calcium Imaging Light Field Microscopy Plenoptic Microscopy Zebrafish Neuroimaging Behavior Image Processing Computer-Assisted Animals Image sensor Zebrafish Brain Chemistry biology Anatomy biology.organism_classification Olfactory Bulb Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Molecular Imaging Key light Microscopy Fluorescence Temporal resolution Larva Calcium Molecular imaging Neuroscience Light field |
| Zdroj: | Europe PubMed Central J. Biomed. Opt. 20:96009 (2015) |
| ISSN: | 1560-2281 |
| Popis: | © 2015 Society of Photo-Optical Instrumentation Engineers (SPIE). Reconstructing a three-dimensional scene from multiple simultaneously acquired perspectives (the light field) is an elegant scanless imaging concept that can exceed the temporal resolution of currently available scanning-based imaging methods for capturing fast cellular processes. We tested the performance of commercially available light field cameras on a fluorescent microscopy setup for monitoring calcium activity in the brain of awake and behaving reporter zebrafish larvae. The plenoptic imaging system could volumetrically resolve diverse neuronal response profiles throughout the zebrafish brain upon stimulation with an aversive odorant. Behavioral responses of the reporter fish could be captured simultaneously together with depth-resolved neuronal activity. Overall, our assessment showed that with some optimizations for fluorescence microscopy applications, commercial light field cameras have the potential of becoming an attractive alternative to custom-built systems to accelerate molecular imaging research on cellular dynamics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|