Live Cell Multicolour Lifetime Imaging Using Genetically Encodable Fluorophores
| Autor: | Tobias Starling, Irene Carlon-Andres, Maro Iliopoulou, David J. Williamson, Sergi Padilla-Parra |
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| Rok vydání: | 2022 |
| Zdroj: | BioRxiv bioRxiv |
| DOI: | 10.1101/2022.10.06.511114 |
| Popis: | Nine fluorescent proteins (FPs) simultaneous imaging has been demonstrated in a single acquisition using fluorescence lifetime imaging microscopy (FLIM) combined with pulsed interleaved excitation (PIE) for three laser lines. We also show how to unmix spectrally similar FPs in a pixel-by-pixel manner with an analytical non-fitting solution. |
| Databáze: | OpenAIRE |
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