Synchronous detection of ebolavirus conserved RNA sequences and ebolavirus-encoded miRNA-like fragment based on a zwitterionic copper (II) metal–organic framework
| Autor: | Gui-Hua Qiu, Bin Sun, Bao-Ping Xie, Xiao-Yan Tang, Jin-Xiang Chen, Zi-Hua Weng, Wen-Jun Duan, Pei-Pei Hu |
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| Rok vydání: | 2018 |
| Předmět: |
Analyte
Stereochemistry Phenanthroline Stacking 02 engineering and technology Molecular Dynamics Simulation 010402 general chemistry medicine.disease_cause 01 natural sciences Analytical Chemistry chemistry.chemical_compound medicine Humans Conserved Sequence Metal-Organic Frameworks Fluorescent Dyes Ebolavirus Base Sequence Chemistry Hydrogen bond Nucleic Acid Hybridization RNA Hemorrhagic Fever Ebola Fluoresceins 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences MicroRNAs Spectrometry Fluorescence RNA Viral DNA Probes 0210 nano-technology Copper DNA |
| Zdroj: | Talanta. 180:396-402 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2017.12.045 |
| Popis: | From a three-dimensional (3D) metal-organic framework (MOF) of {[Cu(Cmdcp)(phen)(H2O)]2·9H2O}n (1, H3CmdcpBr = N-carboxymethyl-(3,5-dicarboxyl)pyridinium bromide, phen = phenanthroline), a sensitive and selective fluorescence sensor has been developed for the simultaneous detection of ebolavirus conserved RNA sequences and ebolavirus-encoded microRNA-like (miRNA-like) fragment. The results from molecular dynamics simulation confirmed that MOF 1 absorbs carboxyfluorescein (FAM)-tagged and 5(6)-carboxyrhodamine, triethylammonium salt (ROX)-tagged probe ss-DNA (probe DNA, P-DNA) by π…π stacking and hydrogen bonding, as well as additional electrostatic interactions to form a sensing platform of P-DNAs@1 with quenched FAM and ROX fluorescence. In the presence of targeted ebolavirus conserved RNA sequences or ebolavirus-encoded miRNA-like fragment, the fluorophore-labeled P-DNA hybridizes with the analyte to give a P-DNA@RNA duplex and released from MOF 1, triggering a fluorescence recovery. Simultaneous detection of two target RNAs has also been realized by single and synchronous fluorescence analysis. The formed sensing platform shows high sensitivity for ebolavirus conserved RNA sequences and ebolavirus-encoded miRNA-like fragment with detection limits at the picomolar level and high selectivity without cross-reaction between the two probes. MOF 1 thus shows the potential as an effective fluorescent sensing platform for the synchronous detection of two ebolavirus-related sequences, and offer improved diagnostic accuracy of Ebola virus disease. |
| Databáze: | OpenAIRE |
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